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EC number: 806-451-7 | CAS number: 42532-60-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 May - 19 June 2020
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: 3M N.B # us278898.001-096
- Expiration date of the lot/batch: 10/30/2021
- Purity: 97.7%
- Physical state: White solid
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under storage conditions: Stable - Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil: A 5 g/L stock solution was made in deionized reverse osmosis water. The test matrix components (5 g/L test substance stock solution, deionized water, and basic test substrate) were mixed evenly and distributed to 4 containers.
- Controls: One blank control of deionized water - Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Earthworm
- Source: Purchased from Jiang Su province Jurong Stock Farm (ID: EF-MKT-191212)
- Weight at test initiation (mean and range, SD): 300 - 600 mg.
ACCLIMATION
- Acclimation period: Quarentined and observed for 14 days
- Acclimation conditions: Earthworms were kept on a substarte of 50% by volume peat, 50% by volume cow manure at 20 ± 2 °C for 7 days prior to test. The test subjects were transfered to artifical test soil (without test substance) 24 hours prior to the test. - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Remarks:
- 10% w/w Sphagnum peat, 20% w/w Kaolinite clay, 70% w/w silica sand
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 18.0 - 18.1°C
- pH:
- 6.10 - 6.72
- Moisture:
- 26.61-29.08 %
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1 liter glass bottles.
- Amount of soil or substrate: 750 grams wet weight of substrate
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: Four
- No. of replicates per control: Four
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): Silica sand 70% w/w, Kaolinite clay 20% w/w, Spahnum peat 10% w/w
- Maximum water holding capacity (in % dry weigth): 58%
- Stability of test material in the medium: Stable
OTHER TEST CONDITIONS
- Photoperiod: Continuous illumination
- Light intensity: 719-761 lux
EFFECT PARAMETERS MEASURED : Mortality, form, behavior and body weight.
VEHICLE CONTROL PERFORMED: No
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2x
- Range finding study: Yes
- Test concentrations: 10, 100, 1000 mg/kg
- Results used to determine the conditions for the definitive study: Yes - Nominal and measured concentrations:
- Nominal concentrations: 10, 22, 48.4, 106, 232, 514, 1129 mg/kg dry artifical soil weight.
- Reference substance (positive control):
- yes
- Remarks:
- Chloroacetamide
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 64.7 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI: 39.8 to 104 mg/kg dw
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- 96.7 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI: 45.2 to 207 mg/kg dw
- Details on results:
- - Mortality at end of exposure period: See Table 1
- Changes in body weight of live adults at end of exposure period: 10.68±2.49% (blank control), 11.14±0.97% (10 mg/kg), 2.94±6.86% (22 mg/kg), -13.37±12.03% (48.4 mg/kg), -15.6±24.94 (106 mg/kg). At 232, 514 and 1129 mg/kg, 100% mortality seen.
- Morphological abnormalities: No abnormalities observed at either 7 or 14 day exposure for the surviving worms in any treatment groups.
- Behavioural abnormalities: No adverse behavioral effects observed at either 7 or 14 day exposure for the surviving worms in any treatment groups. - Results with reference substance (positive control):
- 14 day LC50 = 21.1 mg/kg (95% CI: 18.3-24.4 mg/kg) for chloroacetamide in earthworms from the same batch.
- Reported statistics and error estimates:
- Data was analyzed using Toxcalc v5.0.32 to calculate the 7 and 14 day LC50 values with 95% confidence limits.
- Validity criteria fulfilled:
- yes
- Remarks:
- <10% mortality in controls (0%)
- Conclusions:
- 14 day LC50 of 64.7 mg/kg dw (OECD 207) in Eisenia fetida.
7 day LC50 of 96.7 mg/kg dw (OECD 207) in Eisenia fetida. - Executive summary:
The 14 day LC50 of Heptafluoropropylamide to Eisenia fetida was determined according to OECD 207 guidelines. In the definitive test, nominal concentrations of 10, 22, 48.4, 106, 232, 514, 1129 mg/kg and a blank control, were run using 10 animals per tank, with four replicates per tank. A 14 day LC50 of 64.7 mg/kg (dry artificial soil weight) were found for Eisenia fetida.
The study was well-documented, followed an international standard method and was GLP compliant. The study is considered reliable without restrictions. The results from this study are considered suitable for Risk Assessment, Classification and Labeling, and PBT Analysis
Reference
Table 1. Mortality of E. fetida exposed to the test substance and controls after 7 and 14 days of exposure.
Duration (d) and test concentration |
# of Earthworms |
# dead worms (Rep A) |
# dead worms (Rep B) |
# dead worms (Rep C) |
# dead worms (Rep D) |
Total |
Mortality (%) |
7 d (Blank) |
40 |
0 |
0 |
0 |
0 |
0 |
0 |
14 d (Blank) |
40 |
0 |
0 |
0 |
0 |
0 |
0 |
7 d (10 mg/L) |
40 |
0 |
0 |
0 |
0 |
0 |
0 |
14 d (10 mg/L) |
40 |
0 |
0 |
0 |
0 |
0 |
0 |
7 d (22 mg/L) |
40 |
0 |
0 |
0 |
0 |
0 |
0 |
14 d (22 mg/L) |
40 |
1 |
2 |
2 |
3 |
8 |
20.0 |
7 d (48.4 mg/L) |
40 |
3 |
4 |
4 |
2 |
13 |
32.5 |
14 d (48.4 mg/L) |
40 |
3 |
4 |
5 |
3 |
15 |
37.5 |
7 d (106 mg/L) |
40 |
1 |
4 |
2 |
3 |
10 |
25.0 |
14 d (106 mg/L) |
40 |
3 |
6 |
7 |
5 |
21 |
52.5 |
7 d (232 mg/L) |
40 |
10 |
10 |
10 |
10 |
40 |
100 |
14 d (232 mg/L) |
40 |
/ |
/ |
/ |
/ |
- |
- |
7 d (514 mg/L) |
40 |
10 |
10 |
10 |
10 |
40 |
100 |
14 d (514 mg/L) |
40 |
/ |
/ |
/ |
/ |
- |
- |
7 d (1129 mg/L) |
40 |
10 |
10 |
10 |
10 |
40 |
100 |
14 (1129 mg/L) |
40 |
/ |
/ |
/ |
/ |
- |
- |
/ : no surviving earthworms,
- : not applicable
Description of key information
14 day LC50 of 64.7 mg/kg dw (OECD 207) in Eisenia fetida.
7 day LC50 of 96.7 mg/kg dw (OECD 207) in Eisenia fetida.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 64.7 mg/kg soil dw
Additional information
The 14 day LC50 of Heptafluoropropylamide to Eisenia fetida was determined according to OECD 207 guidelines. In the definitive test, nominal concentrations of 10, 22, 48.4, 106, 232, 514, 1129 mg/kg and a blank control, were run using 10 animals per tank, with four replicates per tank. A 14 day LC50 of 64.7 mg/kg (dry artificial soil weight) were found for Eisenia fetida.
The study was well-documented, followed an international standard method and was GLP compliant. The study is considered reliable without restrictions. The results from this study are considered suitable for Risk Assessment, Classification and Labeling, and PBT Analysis
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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