hydronium;iron(3+);hexahydroxide;disulfate

Additional information on environmental fate and behaviour

Administrative data

Endpoint:

additional information on environmental fate and behaviour

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Scientific publication, thereof only the abstract was accessed since the article is written in Japanese and the subjects was considered too unimportant for translation

Data source

Reference

Reference Type:

publication

Title:

Thiobacillus ferrooxidans. Experimental study on formation of jarosite and ammoniojarosite associated with Thiobacillus ferrooxidans [in Japanese]

Author:

Koiwasaki K, Honbou H, Tazaki K, Mori T

Year:

1993

Bibliographic source:

ISSN 03666611 Chikyu Kagaku (Chigaku Dantai Kenkyukai, The Association for the Geological Collaboration in Japan AGCJ) 47(6):493-506. URL http://ci.nii.ac.jp/naid/110007092862

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Formation of Jarisite crystals was observed in absence and in presence of Thiobacillus ferrooxidans

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Jarosite (K(Fe3(OH)6(SO4)2))

IUPAC Name:

Jarosite (K(Fe3(OH)6(SO4)2))

Constituent 2

Reference substance name:

12207-14-6

Cas Number:

12207-14-6

IUPAC Name:

12207-14-6

Details on test material:

- Name of test material (as cited in study report):
- Molecular formula (as other than submission substance): Fe3-H6-O14-S2.K
- Molecular weight (as other than submission substance): 500.7984 g/mol
- Smiles notation (if other than submission substance): [OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[O-]S(=O)(=O)[O-].[O-]S(=O)(=O)[O-].[K+].[Fe+3].[Fe+3].[Fe+3]
- InChl (if other than submission substance): 1S/3Fe.K.2H2O4S.6H2O/c;;;;2*1-5(2,3)4;;;;;;/h;;;;2*(H2,1,2,3,4);6*1H2/q3*+3;+1;;;;;;;;/p-10

Results and discussion

Any other information on results incl. tables

Bacillus bacterial cells took the form of cross-finger. Both iron and sulphur components precipitated on the cell wall. The iron component took the form of burs, whereas the sulphur component formed rosary-shaped materials. The sizes of iron materials ranged from 0.5 to 2.0 μm in diameter while those of sulphur materials ranged from 0.05 to 0.1μm in diameter. Next, potassium and hydroxyl in 9 K-medium reacted on both iron and sulphur components on the cell wall.

Cubic or platy jarosite, 0.1 to 5.0μm in diameter, was formed within 3 days. Jarosite of final products changed to reform rosary or parallel crystalline materials. Potassium in jarosite was substituted for ammonia to form ammoniojarosite within 5 days.

Jarosite was produced by bacterial mineralization in 9 K-medium within 3 days, and those of ammoniojarosite within 5 days. Without Thiobacillus ferrooxidans, jarosite and ammoniojarosite were produced after 5 or 9 days respectively.

In conclusion the results suggest that Thiobacillus ferrooxidans contributes to the formation of jarosite and ammoniojarosite as catalyst.

Applicant's summary and conclusion

Conclusions:

Thiobacillus ferrooxidans contributes to the formation of jarosite and ammoniojarosite as catalyst.

Executive summary:

Jarosite KFe3(SO4)2(OH)6 and ammoniojarosite NH4Fe3(SO4)2(OH)6 were experimentaly formed in 9 K-medium with gram negative, obligately autotrophic and aerobic Proteobacteria (Thiobacillus ferrooxidans) at 33 ℃ under acidic condition. The product shows characteristic peaks of jarosite at 3.08, 1.99 and 1.83 A and those of ammoniojarosite at 3.09, 5.12, 1.98 and 1.83 A in use X-ray powder diffraction and electron diffraction pattern. Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) observations revealed the crystal growth processes of these minerals.

Bacillus bacterial cells took the form of cross-finger. Both iron and sulphur components precipitated on the cell wall. The iron component took the form of burs, whereas the sulphur component formed rosary-shaped materials. The sizes of iron materials ranged from 0.5 to 2.0 μm in diameter while those of sulphur materials ranged from 0.05 to 0.1μm in diameter. Next, potassium and hydroxyl in 9 K-medium reacted on both iron and sulphur components on the cell wall.

Cubic or platy jarosite, 0.1 to 5.0μm in diameter, was formed within 3 days. Jarosite of final products changed to reform rosary or parallel crystalline materials. Potassium in jarosite was substituted for ammonia to form ammoniojarosite within 5 days.

Jarosite was produced by bacterial mineralization in 9 K-medium within 3 days, and those of ammoniojarosite within 5 days. Without Thiobacillus ferrooxidans, jarosite and ammoniojarosite were produced after 5 or 9 days respectively.

In conclusion the results suggest that Thiobacillus ferrooxidans contributes to the formation of jarosite and ammoniojarosite as catalyst.