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EC number: 221-254-0 | CAS number: 3047-32-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30/07/12-1/08/12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- not required
- Details on sampling:
- No information provided
- Vehicle:
- no
- Details on test solutions:
- A nominal 5000 mg/l stock solution of TMPO was prepared in reverse osmosis water. A nominal 500 mg/l solution of TMPO was prepared by dilution of the 5000 mg/l stock in reverse osmosis water. Test solutions were prepared by addition of a known volume of these stock solutions to a total volume of 100 ml reverse osmosis water, synthetic sewage and activated sludge.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge was obtained from the Totnes Sewage Works, Totnes, Devon, UK, on the day of exposure of the test and reference substance. The activated sludge was settled and supernatent decanted. The settled sludge was fed with 50 ml of OECD syynthetic sewage feed per litre of sludge per day and aerated at room temperature until t was used in the test. The pH was measured at 6.9 and was adjusted to 7.2 prior to use.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- Not applicable
- Hardness:
- No information provided
- Test temperature:
- 20 ±2oC
- pH:
- 7.5-8.4
- Dissolved oxygen:
- No information provided
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentration = 10, 100 and 1000 mg/l
- Details on test conditions:
- See methods below
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- In test flask dosed with TMPO, >10% inhibition was observed in all tested concentrations.
The respiration rate of the abiotic controls was negligible. The 3-hour EC50 was found to be greater than 1000 mg/l. The 3-hour EC20 was determined to be 150 mg/l and the 3-hour NOEC was determined to be less than 10 mg/l. - Results with reference substance (positive control):
- The 3-hour EC50 for the reference substance (3,5-dichlorophenol) was found to be 3.2 mg/l. As this was in the 2 to 25 mg/l recommended range, the test is considered valid.
- Reported statistics and error estimates:
- The per centage inhibition was calculated as follows:
%inhibition = [1-[respiration rate of test flask/mean respiration rate of controls]] x 100 - Validity criteria fulfilled:
- yes
- Conclusions:
- The 3-hour EC50 of TMPO to activated sludge micro-organisms is >1000 mg/l and the 3-hour NOEC is <10 mg/l.
- Executive summary:
A 3-hour OECD 209 activated sludge respiration inhibition test was carried out using trimethylolpropane oxetane. Test solutions were created containing the test substance at concentrations of 10, 100 and 1000 mg/l along with synthetic sewage, activated sludge and reverse osmosis water. 3,5-dichlorophenol was used as a reference substance. The respiration rate of each culture was measured after 3 hours and compared with the mean respiration rate of the controls to determine the percentage inhibition.
The 3 -hour EC50 was found to be greater than 1000 mg/l, while the 3-hour NOEC was found to be less than 10 mg/l.
Reference
Table 1: Inhibition results.
Test substance | Nominal test concentration (mg/l) | pH start | pH end | Respiration rate (mg/L/h) | Percentage inhibition |
Control | Control | 7.5 | 8.1 | 31.6 | - |
3,5 -DCP | 32(abiotic) | 7.5 | 7.4 | - | - |
3,5 -DCP | 32 | 7.5 | 8.3 | 4.3 | 86 |
3,5 -DCP | 10 | 7.4 | 8.4 | 8.7 | 72 |
3,5 -DCP | 3.2 | 7.7 | 8.4 | 15.6 | 50 |
3,5 -DCP | 1.0 | 7.6 | 8.3 | 15.0 | 52 |
Control | Control | 7.7 | 8.1 | 30.8 | - |
TMPO | 1000 (abiotic) | 7.5 | 7.5 | - | - |
TMPO | 1000 | 7.6 | 8.2 | 21.2 | 32 |
TMPO | 1000 | 7.8 | 8.2 | 21.6 | 31 |
TMPO | 100 | 7.7 | 8.1 | 25.5 | 18 |
TMPO | 10 | 7.7 | 8.1 | 27.3 | 12 |
Description of key information
The 3-hour EC50 was found to be >1000 mg/l, thereby exceeding the normal highest dose recommended for such studies (1000 mg/L). Therefore, in the chemical safety assessment performed according to Article 14(3) in connection with Annex I section 3 (Environmental Hazard Assessment) no hazard was identified, therefore no PNEC can be derived.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
A 3-hour OECD 209 activated sludge respiration inhibition test was carried out using 3-ethyloxetane-3-methanol. Test solutions were created containing the test substance at concentrations of 10, 100 and 1000 mg/l along with synthetic sewage, activated sludge and reverse osmosis water. 3,5-dichlorophenol was used as a reference substance. The respiration rate of each culture was measured after 3 hours and compared with the mean respiration rate of the controls to determine the percentage inhibition.
The 3-hour EC50 was found to be greater than 1000 mg/l, while the 3 -hour NOEC was found to be less than 10 mg/l.
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