Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
July 6-8 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: done under GLP and OECD method

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
At 0 and 48 hours. samples were removed from the treatment vessels and anlyzed by the HPLC method. the exposure concentration was achieved.

Test solutions

Vehicle:
no
Details on test solutions:
The test solution was prepared by transferring weighted amounts of A-164220.0 into the glas beakers. The containers were made up to 200 mL with reconsituted water in order to obtain the final concentration of 100 mg/L

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Fill in what you can

TEST ORGANISM
- Common name: Crustacean species
- Source: laboratory breeding


Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
Oberservation of immobilization was made at 0 and 48 hours. Daphnia was considered immobile if not able to swim within 15 seconds of gentle agitation of the test container.

Test conditions

Hardness:
251 mg/L
Test temperature:
monitored during the study
pH:
monitored during the study
Nominal and measured concentrations:
Nominal conc = 100 mg/l
Measured conc = 108.31 mg/l @ 0 hours
Measured conc = 108.18 mg/l @ 48 hours
Details on test conditions:
The values of dissolved oxygen, pH and temperature alwaysreminaed within the acceptable limits.

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 of A-164220.0 at 24 and 48 hours were higher tha 100mg/L