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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
10 Aug - 13 Aug, 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Done under GLP and OECD methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
Version / remarks:
Method C3, Directive 92/69/EEC
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Nominal test concentration: 100mg/L
Mean measured test concentrations at t=0: 103.18mg/L
Mean measured test concentrations at t=72hrs: 101.80mg/L

One control with algal growing medium was also used.
Cell concentrations were determined at 24, 48 and 72 hours after the start of the study.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Culture Centre of Algae and Protozoa, Institute of FreshWater Ecology, Windermere Laboratory, Far Sawrey, Ambleside, Cumbria LA22 0LP, England.

Study design

Test type:
other: aeration
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
23 ± 2°C
Nominal and measured concentrations:
Nominal test concentration: 100mg/L
Mean measured test concentrations at t=0: 103.18mg/L
Mean measured test concentrations at t=72hrs: 101.80mg/L
Details on test conditions:
Algae were cultured in double strength EPA algal assay medium for 72hrs under continuous uniform illumination of approx. 6,000 lux in the spectral range 400 -700 nm.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
EbC50(72h) : >100mg/L measured
ErC50(72h) : >100mg/L measured
NOEC b (72hr): >100mg/L measured
NOEC r (72hr): >100mg/L measured

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EbC50(72h) : >100mg/L measured
ErC50(72h) : >100mg/L measured
NOEC b (72hr): >100mg/L measured
NOEC r (72hr): >100mg/L measured