Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
July 5-9 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: done under GLP and OECD method

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
At the beginning and then at 96hrs for the 22, 48 and 100 % concentrations. Also ran a stability sample concurrent to look for maintenace of test solution concentration.

Test solutions

Vehicle:
no
Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: No auxiliary solvents were required.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Zebra fish: Brachydanio rerio (Cyprinidae species)

7 animals per concentration
weight = 0.27 g
lenght = 3cm

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
Fish inspected at 24, 48, 72 and 96 hours

Test conditions

Hardness:
247 mg/L
Test temperature:
23 oC
pH:
7.8
Dissolved oxygen:
8.4 mg/l
Nominal and measured concentrations:
expected concentration = 100 mg/l @ 0 hours
Measured concentration = 106.91 mg/l @ 0 hours

expected concentration = 100 mg/l @ 24 hours
Measured concentration = 106.66 mg/l @ 24 hours
Nomin
Details on test conditions:
the fish are exposed to artificial lighting with a light period of 16 hours daily. fish are fasted during test.

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The LC50at each observation (24,48, 72 and 96 hours) was higher than 100 mg/l.+
No animals of the control or treated group showed visible signs of abnormalities.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 for the test substance is estimated to be greater than 100 mg/l