3-hydroxypropiononitrile

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions No information about controls

Data source

Reference

Reference Type:

secondary source

Title:

Toxicity studies of acetone cyanohydrin and ethylene cyanohydrin.

Author:

Sunderman WF, Kincaid JF

Year:

1953

Bibliographic source:

Archives of Industrial Hygiene and Occupational Medicine 8: 371-376

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Method: Patch Test

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-hydroxypropiononitrile

EC Number:

203-704-8

EC Name:

3-hydroxypropiononitrile

Cas Number:

109-78-4

Molecular formula:

C3H5NO

IUPAC Name:

3-hydroxypropanenitrile

Details on test material:

- Name of test material (as cited in study report): Ethylene cyanohydrin
- InChl (if other than submission substance):
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: aliphatic nitrile
- Physical state: liquid
- Analytical purity: 92.5% by analysis
- Impurities (identity and concentrations): The balance of the material was presumed to be largely water.

Test animals

Species:

guinea pig

Strain:

not specified

Sex:

not specified

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: 2.5 squares (cm2)
- Type of wrap if used: cheese cloth (patch), covered with an adhesive tape, completely encircling the animal.

REMOVAL OF TEST SUBSTANCE
-Application of the test substance via patches until death occurred or until recovery appeared to be complete: 24h time of exposure.

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5, 0.2, 0.1 ml of Ethylene cyanohydrin.

Duration of exposure:

24h time of exposure

Doses:

0.5, 0.2, 0.1 ml of Ethylene cyanohydrin

No. of animals per sex per dose:

6

Control animals:

not specified

Details on study design:

The abdominal surfaces of the guinea pigs were shaved with an electric razor to avoid skin cuts or abrasions. 
The test substance was applied to 2.5 cm² of cheese cloth, being used for the application dosage of 0.1, 0.2, 0.5 ml. 
These prepared patches were applied to the shaved area topside down and they were covered with a 5 cm band of adhesive tape
completely encircling the animals. Observation was maintained until death occurred or until recovery appeared to be complete.

Results and discussion

Preliminary study:

no data

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

> 0.5 mL/kg bw

Remarks on result:

other: Ethylene cyanhydrin did not effect any test animal; for this reason no LD50 value could be determined.

Mortality:

none

Clinical signs:

none

Body weight:

no data

Gross pathology:

no data

Any other information on results incl. tables

After application of 0.5 ml Ethylene cyanohydrin by the patch test technique as described above, to six animals, there appeared to be some pain shortly after treatment, but no other symptoms were observed.

Examination 24h later showed little if any skin irritation (and no mortalitys).

This result leads to the conclusion, that Ethylene cyanohydrin has a low toxicity by skin penetration.

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

Under the above mentioned test conditions Ethylene cyanohydrin is classified as pratically nontoxic via skin penetration.