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EC number: 203-704-8 | CAS number: 109-78-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30-0kt-1989 - 02-Nov-1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-hydroxypropiononitrile
- EC Number:
- 203-704-8
- EC Name:
- 3-hydroxypropiononitrile
- Cas Number:
- 109-78-4
- Molecular formula:
- C3H5NO
- IUPAC Name:
- 3-hydroxypropanenitrile
- Details on test material:
- - Name of test material (as cited in study report): 2-Cyanoethanol
- Analytic purity: 99.08%
- Storage conditions: cool, dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- - Sampling method: samples of activated sludge were withdrawn from a sewage plant mainly fed with domestic sewage
- Activated sludge was aerated during transportation to the laboratory
- Concentration(suspended solids): adjusted to 3g/l
- Temp: 20 ±2 °C
- pH: 6-8 (adjustment was not necessary)
- Sample storage conditions before analysis: test vessels are placed in a temp controlled room (20 ±2°C)
Test solutions
- Details on test solutions:
- - Concentrations: 1, 10, 100, 10000 mg/l (test substance)
- Controls one blanc control with inoculum but without test material, one physico-chemical control (with test - respectively reference- substance,
but without activated sludge, to determine the chemical oxygen consumption)
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 180 min
- Remarks on exposure duration:
- Determination of the EC50 after 30 min exposure.
Test conditions
- Test temperature:
- 20 ±2°C
- pH:
- 6.0 - 8.0
- Nominal and measured concentrations:
- Nominal: 1, 10, 100, 1000 and 10000 mg/L
- Details on test conditions:
- - Test vessel: each prepared with 16 ml synthetic sewage, dilution water to obtain a volume of 500ml and different amounts of test
material - Renewal rate of test solution (frequency/flow rate): no flowrate, vessels were stirred and aerated with an aeration rate of 1l/min. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid?: Yes. 50% effect concentration(EC) for 180 min contact time lays between 5-10 mg/l, in case of 30 min
contact time 50% EC is reached at a substance level between 10-30 mg/l .
- Relevant effect levels: for the test substance, effect level for 50% inhibition was not obtained for 30 min contact time , therefore extrapolated EC 50: > 10000mg/l
Any other information on results incl. tables
The oxygen consumption (mg/l x h) was calculated from the linear part of the curves of the oxygen concentration versus time.
The percentage inhibition was calculated at each concentration as follows:
I(%) =[1- (Rs - Rpc)/ Rc)]x100
I = percentage inhibition of oxygen consumption
Rs = oxygene consumption rate by the test mixture
Rc = oxygen consumption rate by the blanc control
Rpc= oxygen uptake rate by the physico-chemical control
Table 2: Oxygen uptake and inhibition (%) of the test substance 2-Cyanoethanol after a contact time of 30 min
O2 uptake (mg/l*h) | Inhibition (%) | |
Control C | 61.60 | |
1 mg/l | 62.60 | -2 |
10 mg/l | 63.20 | -3 |
100 mg/l | 60.20 | 2 |
1000 mg/l | 61.80 | 0 |
10000 mg/l | 58.20 | 6 |
phsico-chemical control | 0.00 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The EC50 of 3,5-Dichlorphenol was in the accepted range of 5 to 30 mg/L.
- Executive summary:
Even the highest concentration (10 g/l) of the test substance, ethylencyanhydrin, caused no significant raise of the inhibition rate.
The O2-uptake of the activated sludge was reduced at a contact time about 30 min for only ~ 6%.
Therefore, to obtain 50% inhibition of respiration as measurable effect, substance concentration must be >10 g/l.
That means, EC50 (ethylencyanhydrin) is > 10 g/l,as it is shown by extrapolating the curve of function (inhibition of respiration vs concentration of ethylencyanhydrin) to higher values.
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