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EC number: 203-783-9 | CAS number: 110-61-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- mammalian erythrocyte micronucleus test
Test material
- Reference substance name:
- Succinonitrile
- EC Number:
- 203-783-9
- EC Name:
- Succinonitrile
- Cas Number:
- 110-61-2
- Molecular formula:
- C4H4N2
- IUPAC Name:
- butanedinitrile
- Details on test material:
- - Chemical source: Aldrich Chemical
- Purity: 97% (according to label)
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: DSM Engineering Plastics B.V. lotnumber SN0620140520
- Expiration date of the lot/batch: 2016.07.01
- Purity test date: 99.93%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Store in a cool area (IO-20°C). Containers that have been opened must be filled with dry nitrogen for at least 1min and then sealed. Be careful not to import any water or impurities during the filling and sealing course.
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: Easily soluble in cold water
FORM AS APPLIED IN THE TEST white waxy solid
Test animals
- Species:
- mouse
- Strain:
- ICR
- Sex:
- male
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle(s)/solvent(s) used:water
- Justification for choice of solvent/vehicle: due to the good solubility of the solvent in water
- Concentration of test material in vehicle:
- Amount of vehicle (if gavage or dermal):
- Type and concentration of dispersant aid (if powder):
- Lot/batch no. (if required):
- Purity:
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Remarks on result:
- other: body weight decrease is observed in the lower dose group .
Applicant's summary and conclusion
- Conclusions:
- Conclusion. Under the conditions of this study, the results were negative, so the
study suggested that succinonitrile did not induce an increase of the incidence of
micronucleated PCE in mice. - Executive summary:
Introduction. This study was conducted to detect the possibility that Succinonitrile could induce the increase of the incidence of micronucleated polychromatic erythrocytes (PCE) in mice, in order to provide genotoxicity related data for the test item. The method was designed to be compatible with the requirements of OECD Guideline for the Testing of Chemicals, 474 “Mammalian Erythocyte Micronucleus Test” (2014).
Method. This study was conducted in ICR mice. All animals were SPF grade. According to the related information about the test item and the results of the preliminary test, in the micronucleus test, the animals were treated at 50, 25 and
12.5mg/kg.bw. Negative control (N G, Ultrapure water) group and positive control (Cyc10phOSphamide, CP, 50mg/kg) group were performed at the same time with the same method. All animals were randomly grouped based on body weight with 7 male animals in each group. Mice were administered the test item orally by gavage twice, with 24 hours interval between doses. All animals were sacrificed by cervical dislocation nearly 19 hours after the last administration. Bone marrow
from stemum was harvested. Bone marrow smear was prepared and analysed with microscope. The data were evaluated with t-test (two-tail) in Excel.
Results. During the test, a notable decrease in the body weights of the animals was found in 12.5mg/1cg.bw dose group as comparing with the concurrent NG approximate 24h after the first administration. The clinical observation results
showed that three mice were sluggish in the 50mg/kg.bw dose group after the first administration or the last administration, and one mouse had hematuria before being sacrificed.
The frequencies of micronucleated PCB were 1.2 +/-20.3%n in the NG group, 1.4 +/-0.2%o in the 12.5mg/kg.bw group, 1.3 +/-0.7% in the 25mg/kg.bw group, 1.6 +/-1.0% in the 50mg/kg.bw group and 24.4 +/-5.5%o in the CP group. No statistically significant difference (P>0.05) in the incidence of micronucleated PCE was observed in all
treated groups as compared with NG. At the same time, a statistically significant difference (P<0.01) in the incidence of micronucleated PCE was observed in the CP group as compared with NG. Furthermore, the PCE/RBC ratios in all treated
groups and positive control group were more than twenty percent of the ratio in NG.
Conclusion. Under the conditions of this study, the results were negative, so the study suggested that succinonitrile did not induce an increase of the incidence of micronucleated PCE in mice.
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