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EC number: 203-783-9 | CAS number: 110-61-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
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- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Nanomaterial pour density
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- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
An inhalation toxicity study and an acute dermal toxicity study were performed in accordance with the current OECD guideline. Both studies are reliable with restrictions.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- yes
- Remarks:
- Animals where housed with 2 in a cage during acclimatisation. The animals showed normal during the acclimatization period, it was considered not to affect the animals and study results.
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: DSM Engineering Plastics B.V. lotnumber SN0620140520
- Expiration date of the lot/batch: 2016.07.01
- Purity test date: 99.93%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Store in a cool area (IO-20°C). Containers that have been opened must be filled with dry nitrogen for at least 1min and then sealed. Be careful not to import any water or impurities during the filling and sealing course.
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: Easily soluble in cold water
FORM AS APPLIED IN THE TEST white waxy solid - Species:
- rat
- Strain:
- other: Sprague Dawley (SD)
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Supplier: Beijing Vital River Laboratory Animal Technology Co., Ltd.
License No.: SCXK (Jing) 2012—0001, Qualification: 11400700112907.
- Age at study initiation: 51-60 days on arrival, in the range of 8-12 weeks at the commencement of each
animal’s dosing.
- Weight at study initiation: 182-222g
- Housing:Animals were raised
in suspended, stainless steel cages (L32.0cm >(L167.0cmXW70.0cm> Animals were housed individually during the test.
- Diet : diet with complete nutrition supplied by Beijing keaoxieli Feed Co., Ltd. Product License No: SCXKUing) 2012-0001, Batch NO. 15063223).
- Water (e.g. ad libitum): purified water (by HT-R01000 purity system.)
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22.5-24.5 °C
- Humidity (%): 45%-70%
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light): lighting sequence was 12 hours light, 12 hours dark.
- Route of administration:
- oral: gavage
- Doses:
- 300 mg/kg BW and 2000 mg/kg BW
- No. of animals per sex per dose:
- 3
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: 30 min and at l, 2 and 4 hours after dosing and then once each day for up to 14 days.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:changes in skin and fur, eyes and mucous membranes, and also respiratory, circulatory, autonomic and central nervous systems, and somatomotor activity and behaviour pattern. Attention had been directed to observations of tremors, convulsions, salivation, diarrhoea, lethargy, sleep and coma. - Preliminary study:
- LD50 is 488.7 mg/kg b.w. for rats was available. Based on that value the test doses were selected.
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- >= 300 - <= 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- Mortality
Dose Level-The first dosing (300mg/kg): One animal was dead on Day 2 after dosing. Dose Level-The second dosing (300mg/kg): There were no deaths or moribund during the test. Dose Level-The third dosing (2000mg/kg): All animals were dead in 4h after dosing. Mortality summary results were given in appendix Table 1. - Clinical signs:
- other: Dose Level-The first dosing (300mg/kg): Animals showed decrease in locomotor activity, emaciation, and soiled perinea region after dosing. Dose Level-The second dosing (3 00mg/kg): There were no abnormal findings after dosing from the first day until the end
- Gross pathology:
- Necropsy
No abnormalities were found at necropsy.
Necropsy results see appendix table 4. - Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- Based on the results, the acute oral LDso in the rats for Succinonitrile was estimated to be among 300 mg/kg b.w. - 2000 mg/kg b.w..
According to the GHS’s classification criteria for acute oral toxicity, the test item was classified as Category 4. - Executive summary:
Introduction: The study was performed to assess the acute oral toxicity of Succinonitrile in Sprague Dawley rats. The method was designed to meet the OECD Guideline for Testing of Chemicals: Acute Oral Toxicity-Acute Toxic Class Method (TG423, 2001).
Method: The test item was tested using a stepwise procedure, each group using three female animals. The first step dosing and second step dosing were 300 mg/kg and the third step dosing was ZOOOmg/kg. Clinical observations and bodyweight were monitored during the study. All animals were subjected to a gross necropsy.
Results:
Mortality
Dose Level-The first dosing (300mg/kg): One animal was dead on Day 2 after dosing.
Dose Level-The second dosing (300mg/kg): There were no deaths or moribund during
the test.
Dose Level-The third dosing (2000mg/kg): All animals were dead in 4h after dosing.
Clinical observations
Dose Level-The first dosing (300mg/kg): Animals showed decrease in locomotor
activity, emaciation, and soiled perinea region after dosing.
Dose Level-The second dosing (3 00mg/kg): There were no abnormal findings after
dosing from the first day until the end of the test.
Dose Level-The third dosing (2000mg/kg): Animals showed decrease in locomotor
activity, emaciation, secretion around mouth and nose, semi-closed eyes, weak breath,
soiled fur around the mouth and nose after dosing.
Body Weight
All surviving animals showed a increase in body weight during the first week, but most of the surviving animals showed a decrease during the second week.
Necropsy
No abnormalities were found at necropsy.
Conclusion:
Based on the results, the acute oral LDso in the rats for Succinonitrile was estimated to be among 300 mg/kg b.w. - 2000 mg/kg b.w.. According to the GHS’s classification criteria for acute oral toxicity, the test item was classified as, Category 4.
Reference
Table 1. Mortality summary results | |||||||||||||||||||
Dosing sequence |
Dose (mg/kg) |
sex | Animal amounth |
Time (d) | Mortality | ||||||||||||||
0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |||||
1 | 300 | Female | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1/3 |
2 | 300 | Female | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0/3 |
3 | 2000 | Female | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3/3 |
Table 2. Individual Clinical observations | |||||||||||||||||||
Dose (mg/kg) |
Animal ID | Symptons After Dosing (Hours) |
Symptoms During Period After Dosing (Days) |
||||||||||||||||
0.5 | 1 | 2 | 4 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | ||
300 | 2100 | N | N | N | N | S | E1D | - | - | - | - | - | - | - | - | - | - | - | - |
2101 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
2102 | N | N | N | N | H | N | N | N | N | N | N | N | N | N | N | N | N | N | |
300 | 2200 | N | N | N | N | N | E1D | - | - | - | - | - | - | - | - | - | - | - | - |
2201 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
2202 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
300 | 2300 | N | SE | SE Se | FD | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
2301 | N | SE | SE SeW | FD | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
2302 | N | N | SE | FD | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
Note: N=no sign; S=decrease in locomotor activity; E1=emaciation, E=secretion around mouth and nouse, Se-semi closed eyes, W=weak breath, D=death, F=soiled fur around the mouth and nouse, H=soiled perinea region. |
Table 3. Individual and Mean Body Weights | |||||||||
Dose (mg/kg) |
Dose (mg/kg) |
Animal ID | Body Weight (g) at Day | Body Weight Gain (g) During Week | |||||
Grouping | Day0 | Day7 | Day14 | At Death | 1 | 2 | |||
300 | Female | 2100 | 204 | 191 | - | - | 149 | - | - |
2101 | 233 | 212 | 246 | 268 | - | 34 | 22 | ||
2102 | 226 | 209 | 255 | 263 | - | 46 | 8 | ||
Mean +/- SD | 221 +/- 15 | 204 +/- 11 | 251 +/- 6 | 266 +/- 4 | - | 40 +/- 8 | 15 +/- 10 | ||
N | 3 | 3 | 2 | 2 | 1 | 2 | 2 | ||
300 | Female | 2200 | 201 | 183 | - | - | 149 | - | - |
2201 | 219 | 212 | 246 | 268 | - | 34 | 22 | ||
2202 | 225 | 209 | 255 | 263 | - | 46 | 8 | ||
Mean +/- SD | 215+/- 12 | 199 +/- 15 | 245 +/- 16 | 226 +/- 11 | - | 46 +/- 9 | -20+/-6 | ||
N | 3 | 3 | 3 | 3 | - | 3 | 3 | ||
2000 | Female | 2300 | 196 | 191 | - | - | 184.53 | - | - |
2301 | 216 | 212 | - | - | 199.86 | - | - | ||
2302 | 222 | 209 | - | - | 203.18 | - | - | ||
Mean +/- SD | 211 +/- 14 | 198 +/- 7 | - | - | - | - | - | ||
N | 3 | 3 | 0 | 0 | 3 | 2 | 2 | ||
Note: body weight(g), N=Number of animals per group, SD= Standard deviation |
Table 4. Indivudual Negroscopy Findings | ||||
Animal ID | sex | Dose (mg/kg) |
Death time | Findings |
2100 | Female | 300 | Day2 | No abnormalities |
2101 | Female | 300 | Sheduled Negroscopy | No abnormalities |
2102 | Female | 300 | Sheduled Negroscopy | No abnormalities |
2200 | Female | 300 | Sheduled Negroscopy | No abnormalities |
2201 | Female | 300 | Sheduled Negroscopy | No abnormalities |
2202 | Female | 300 | Sheduled Negroscopy | No abnormalities |
2300 | Female | 2000 | Day 0 | No abnormalities |
2301 | Female | 2000 | Day 0 | No abnormalities |
2302 | Female | 2000 | Day 0 | No abnormalities |
Endpoint conclusion
- Dose descriptor:
- LD50
- Value:
- 300 mg/kg bw
Acute toxicity: via inhalation route
Endpoint conclusion
- Dose descriptor:
- discriminating conc.
- Value:
- 2 670 mg/m³ air
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Version / remarks:
- Acute Dermal Toxicity (TG402, adopted 1987).
- GLP compliance:
- yes (incl. QA statement)
- Limit test:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: DSM Engineering Plastics B.V. lotnumber SN0620140520
- Expiration date of the lot/batch: 2016.07.01
- Purity test date: 99.93%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Store in a cool area (IO-20°C). Containers that have been opened must be filled with dry nitrogen for at least 1min and then sealed. Be careful not to import any water or impurities during the filling and sealing course.
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: Easily soluble in cold water
FORM AS APPLIED IN THE TEST white waxy solid - Species:
- rat
- Strain:
- other: Sprague Dawley (SD)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source:
- Females (if applicable) nulliparous and non-pregnant: yes
- Age at study initiation: 56-65 days on arrival, in the range of 8-12 weeks at the commencement of each animal’s dosing.
- Weight at study initiation: Body weight range: Males: 23 5-270g. Females: 225-235g, +/- 20% of the mean body weight at grouping.
-
- Housing: Animals were housed individually during the test.
- Diet : provided sterilized diet with complete nutrition supplied by Beijing keaoxieli Feed
- Water : Purified water
- Acclimation period: 5 days prior to the test
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22.0-24.5"C
- Humidity (%): 45%-70%
- Photoperiod (hrs dark / hrs light): light cycle was 12 hour light/ 12 hour dark. - Type of coverage:
- not specified
- Vehicle:
- water
- Details on dermal exposure:
- TEST SITE
- Area of exposure: fur removed from dorsal area by shaving, clear area 40cm2. A gauze 5cm x 5.50m is used
- Type of wrap if used: small piece of white medical tape(non-irritating)
REMOVAL OF TEST SUBSTANCE
- Washing (if done): esidual test item was
removed by cotton wool soaked in water.
- Time after start of exposure: approximated 24 hour
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg/kg
- For solids, paste formed: yes
VEHICLE
- Amount(s) applied (volume or weight with unit): test item was moistened with the vehicle
- Purity: purified water - Duration of exposure:
- Approximately 24 hours
- Doses:
- 2000
- No. of animals per sex per dose:
- 5 Male
5 female - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: Clinical observations were made during the first 30 min, and at 1, 2 and 4 hours after dosing and then once each day for up to 14 days.
- Frequency of observations and weighing: ndividual weights of animals were determined at grouping, Day 0(day of dosing), Day7 and Dayl4
- Necropsy of survivors performed: yes
- Other examinations performed: Careful observation and record of animal fur changes, eyes and mucosa, respiratory, circulatory, nervous system, particularly limb activity and behavior change were made. Particular attention was directed to observations of tremor, convulsions, salivation, diarrhea, lethargy, sleep and coma. - Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- There were no deaths or moribund during the test.
- Clinical signs:
- other: There were no abnormal findings in all animals after dosing from the first day until the end of the test.
- Gross pathology:
- No abnormalities were found at necropsy
- Other findings:
- Skin Reactions: There were no signs of dermal irritation.
- Interpretation of results:
- other: Category 5 or unclassified.
- Conclusions:
- Conclusion: Based on the results, the acute dermal LD50 in rats for Succinonitrile was as follows:
Male rats :>2000mg/kg b.w.
Female rats :>2000mg/kg b.w.
According to the GHS’s classification criteria of acute dermal toxicity, the test item was classified as Category 5 or unclassified. - Executive summary:
Introduction: The study was performed to assess the acute dermal toxicity of
Succinonitrile in Sprague Dawley rats. The method was designed to meet the OECD
Guideline for Testing of Chemicals: Acute Dermal Toxicity (TG402, adopted 1987).
Method: A limit test at one dose level of 2000mg/kg body weight was carried out in a
group of 10 animals (5 males and 5 females). Clinical observations were made during
the first 30 min, and at 1, 2 and 4 hours after dosing and then once each day for up to 14
days. Individual weights of animals were determined at grouping, Day 0(day of dosing),
Day7 and Dayl4. At the end of the test, a gross necropsy was performed on all animals
under test.
Results:
Mortality
There were no deaths or moribund during the test.
Clinical observations
There were no abnormal findings in all animals after dosing from the first day until the
end of the test.
Skin Reactions
There were no signs of dermal irritation.
Body Weight
One male and three female animals showed a decrease in body weight during the first
week. But overall also these animals showed growth during the second week. The
results indicated that the body weight gain of animals showed growth.
Necropsy
No abnormalities were found at necropsy.
Conclusion: Based on the results, the acute dermal LD50 in rats for Succinonitrile was
as follows:
Male rats :>2000mg/kg b.w.
Female rats :>2000mg/kg b.w.
According to the GHS’s classification criteria of acute dermal toxicity, the test item was
classified as Category 5 or unclassified.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
Additional information
An inhalation toxicity study was performed with 5 rats/sex in accordance with the current OECD guideline. The rats were exposed whole body to the substance by aerosols at the maximal attainable concentration. None of the animals died during the exposure period, but three males died within 48 hours after termination of the exposure. It was concluded that the 4-hour LC50 was higher than 2.67 mg/l of air. Since the LC50 was found to be higher than the highest attainable concentration, the substance does not have to be classified for inhalation toxicity according to CLP Regulation (EC) 1272/2008.
Acute dermal toxicity study with Sprague Dawley:
A limit test at one dose level of 2000mg/kg body weight was carried out in a group of 10 animals (5 males and 5 females). Clinical observations were made during the first 30 min, and at 1, 2 and 4 hours after dosing and then once each day for up to 14
days. Individual weights of animals were determined at grouping, Day 0(day of dosing), Day7 and Dayl4. At the end of the test, a gross necropsy was performed on all animals under test.
Based on the results, the acute dermal LD50 in rats for Succinonitrile was as follows:
Male rats :>2000mg/kg b.w.
Female rats :>2000mg/kg b.w.
According to the CLP Regulation (EC) 1272/2008 the test item is unclassified .
Justification for classification or non-classification
Based on the above inhalation toxicity study, the substance does not have to be classified for inhalation toxicity.
Based on the above acute dermal toxicity study, the substance has to be classified for acute dermal toxicity in category 3 according to CLP Regulation (EC) No 1272/2008.
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