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Diss Factsheets
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EC number: 601-017-1 | CAS number: 110964-79-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996-02-02 to 1996-02-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well performed OECD and GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4-methanesulfonyl-2-nitrobenzoic acid
- EC Number:
- 601-017-1
- Cas Number:
- 110964-79-9
- Molecular formula:
- C8 H7 N1 O6 S1
- IUPAC Name:
- 4-methanesulfonyl-2-nitrobenzoic acid
- Details on test material:
- - Name of test material (as cited in study report): MNBA (2-nitro-4-methylsulfonyl benzoic acid)
- Physical state: solid
- Analytical purity: 97.0 % (w/w)
- Lot/batch No.: WRC15483-30-1
- Storage condition of test material: ambient temperature in the dark
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA1535, TA1537, TA98 and TA100, Escherichia coli WP2P and WP2P uvrA
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 was prepared from male Sprague Dawley rats, dosed once daily (by oral gavage) for 3 days with a combined Phenobarbital (80mg/kg bodyweight) and P-Naphthoflavone (lOOmg/kg) corn oil solution.
- Test concentrations with justification for top dose:
- 100, 200, 500, 1000, 2500, 5000 (MICROGRAMS/PLATE)
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Acridine Mutagen ICR191, 2-Aminoanthracene, Daunomycin HC1, N-Ethyl-N'-nitro-N-nitrosoguanidine, Mitomycin C, Sodium Azide
- Evaluation criteria:
- A positive response in an individual experiment is achieved when one or both of the following criteria are met:
a) a statistically significant dose-related increase in the mean number of revertant colonies is obtained;
b) a two-fold or greater increase in the mean number of revertant colonies (over that observed for the concurrent solvent control plates) which is statistically significant, is observed at one or more concentrations.
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium TA1535, TA1537, TA98 and TA100, Escherichia coli WP2P and WP2P uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Plate incorporation 2500 µg: TA 1535 -S9, TA 1537 -S9, TA 98 -S9, TA 100 -S9, WP2P -S9 / 5000 µg: WP2P uvra -S9 Pre-incubation 5000 µg: TA 1535 -S9, TA 1537 -S9, TA 100 -S9, WP2P -S9, WP2P uvra -S9
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Under the conditions of this assay, MNBA gave a negative, ie non-mutagenic response in S.typhimurium strains TA1535, TA1537, TA98 and TA100 and E.coli strains WP2P and WP2P uvrA in both the presence and absence of S9-mix. substances - Executive summary:
MNBA (2-nitro-4-methylsulfonyl benzoic acid) was evaluated in a bacterial mutagenicity assay over a range of concentrations using four strains of Salmonella typhimurium (TA1535, TA1537, TA98 and TA100) and two strains of Escherichia coli (WP2P and WP2P uvrA) in the presence and absence of a rat liver-derived metabolic activation system (S9-mix), following Plate incorporation and Pre-incubation potocols.
In two separate experiments, the test substance did not induce any significant, reproducible increases in the observed numbers of revertant colonies in any of the tester strains used, either in the presence or absence of S9-mix. The sensitivity of the test system, and the metabolic activity of the S9-mix, were clearly demonstrated by the increases in the numbers of revertant colonies induced by positive control substances.
It is concluded that, under the conditions of this assay, MNBA gave a negative, ie non-mutagenic response in S.typhimurium strains TA1535, TA1537, TA98 and TA100 and E.coli strains WP2P and WP2P uvrA in both the presence and absence of S9-mix. substances
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