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Physical & Chemical properties

Partition coefficient

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Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report Date:
2016

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method A.8 (Partition Coefficient)
Qualifier:
according to
Guideline:
OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)
Qualifier:
according to
Guideline:
EPA OPPTS 830.7550 (Partition Coefficient, n-octanol / H2O, Shake Flask Method)
GLP compliance:
yes
Type of method:
flask method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Name of test material (as cited in study report): Ethyl Ascorbic acid
Physical state: White to very pale yellow crystalline powder
Test item storage: In freezer (≤ -15°C) desiccated
Stability: Stable under storage conditions until 21 April 2018 (retest date)

Study design

Analytical method:
high-performance liquid chromatography

Results and discussion

Partition coefficient
Type:
log Pow
Partition coefficient:
-0.8
Temp.:
20 °C
pH:
7

Any other information on results incl. tables

Main study - shake flask method

The results of the shake flask method are given in the table.

The analysed concentration of the stock solution was 1.90 mg/l. Based on this the nominal quantity of the test item in the test samples was 3.8 μg. The recoveries were determined according to this value. The log Pow values were within the criterion range of ± 0.3 log units. Based on this, the Pow and log Pow of the test item is given as the mean value of all measurements.

No test item was detected in the pre-treated samples from the blank mixture.

Main study: Pow of the test item

Ratio

n-octanol: water [v:v]

Analysed concentration

 

Pow

 

log Pow

pH

n-octanol

[mg/l]

water

[mg/l]

individual

mean

1.1

0.0929

0.592

1.6E-01

1.5E-01

-0.8

6.9

0.0925

0.606

1.5E-01

 

-0.8

6.9

1.2

0.0767

0.514

1.5E-01

1.5E-01

-0.8

6.9

0.0744

0.471

1.6E-01

 

-0.8

7.1

1.3

0.121

0.777

1.6E-01

1.5E-01

-0.8

6.7

0.121

0.794

1.5E-01

 

-0.8

6.7

Mean

1.5E-01

 

-0.8

 

standard deviation

3.3E-03

 

Conclusion

The shake flask method at neutral pH was applied for the determination of the partition coefficient (Pow) of Ethyl Ascorbic acid.

The Pow value of the test item at 20°C was 1.5E-01.

It corresponds with a log Pow value of -0.8.

Applicant's summary and conclusion