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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from J-check database
Qualifier:
according to
Guideline:
other: Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"
Principles of method if other than guideline:
Bioaccumulation test of a chemical substance in fish or shellfish was determined.
GLP compliance:
not specified
Radiolabelling:
not specified
Details on sampling:
No data available
Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle: Tween 80,
- Concentration of vehicle: 1st Concentration area: 1 ppm(w/v),
2nd Concentration area: 0.1 ppm(w/v)
Test organisms (species):
Cyprinus carpio
Details on test organisms:
No data available
Route of exposure:
aqueous
Test type:
static
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
8 wk
Hardness:
No data available
Test temperature:
No data available
pH:
No data available
Dissolved oxygen:
No data available
TOC:
No data available
Salinity:
No data available
Conductivity:
No data available
Details on test conditions:
RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations : TLm(48h) 5.05 ppm(w/v), Rice fish (Oryzias latipes)
Nominal and measured concentrations:
- Nominal concentrations
- 1st Concentration area: 0.1 ppm(w/v),
- 2nd Concentration area: 0.01 ppm(w/v)
Reference substance (positive control):
not specified
Details on estimation of bioconcentration:
No data available
Lipid content:
4.6 %
Remarks on result:
other: E. G. Bligh and W. J. Dyer, Can. J. Biochem, Physiol., 37, 911 (1959)
Conc. / dose:
0.1 other: ppm(w/v)
Type:
BCF
Value:
> 34 - <= 143 L/kg
Basis:
total lipid content
Time of plateau:
8 wk
Calculation basis:
other: No data
Remarks on result:
other: other details not available
Conc. / dose:
0.01 other: ppm(w/v)
Type:
BCF
Value:
> 38 - <= 235 L/kg
Basis:
total lipid content
Time of plateau:
8 wk
Calculation basis:
other: No data
Remarks on result:
other: other details not available
Details on kinetic parameters:
No data available
Metabolites:
No data available
Results with reference substance (positive control):
No data available
Details on results:
No data available
Reported statistics:
No data available

Bioconcentration test results:

 

 

2 w

3 w

4 w

6 w

 8 W

1st concentration area

Test substance concentration (ppb(w/v) )

93.0

90.6

88.8

93.9

 89.1

Bioconcentration factor (L/kg)

34

51, 117

132

105, 143

117, 66 

36

84

100

59

35 

2nd concentration area

Test substance concentration (ppb(w/v) )

9.56

9.07

9.03

9.10

8.54 

Bioconcentration factor (L/kg)

109

-

84

124, 127

 94, 235

38

-

76

53

 74
Validity criteria fulfilled:
not specified
Conclusions:
The bioconcentration factor (BCF) for substance was determined according static fish test. The BCF value was observed to be 34-143 L/kg at dose concentration 0.1 ppm(w/v) and 38- 235 L/kg at dose concentration 0.01 ppm(w/v) on test organism Cyprinus carpio during 8 weeks period.
Executive summary:

Bioaccumulation test was conducted for 8 weeks by using test guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances" for determination the bioconcentration factor (BCF) of substance on test organism Cyprinus carpio.

The recovery ratio is given- Test water: 1st concentration area: 91.4 %, 2nd concentration area: 84.1 %, Fish: 73.5 %, Vehicle used as Tween 80 with concentrations 1st Concentration area: 1 ppm(w/v), 2nd Concentration area: 0.1 ppm(w/v) respectively.

And Nominal concentrations used in the study as - 1st Concentration area: 0.1 ppm(w/v), 2nd Concentration area : 0.01 ppm(w/v) and Range finding study was carried out on Rice fish (Oryzias latipes) and the 48 hr TLm(48h) 5.05 ppm(w/v). 

Thus according to static Fish Test the BCF value for test substance was observed to be 34-143 L/kg at dose concentration 0.1 ppm(w/v) and 38- 235 L/kg at dose concentration 0.01 ppm(w/v) on test organism Cyprinus carpio during 8 weeks period.

These BCF values indicate that the substance is not bioaccumulative in fish and food chain.

Description of key information

Bioaccumulation test was conducted for 8 weeks by using test guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"for determination the bioconcentration factor (BCF) of substance on test organism Cyprinus carpio.

The recovery ratio is given- Test water: 1st concentration area: 91.4 %, 2nd concentration area: 84.1 %, Fish: 73.5 %, Vehicle used as Tween 80 with concentrations 1st Concentration area: 1 ppm(w/v), 2nd Concentration area: 0.1 ppm(w/v) respectively.

And Nominal concentrations used in the study as - 1st Concentration area: 0.1 ppm(w/v), 2nd Concentration area : 0.01 ppm(w/v) and Range finding study was carried out on Rice fish (Oryzias latipes) and the 48 hr TLm(48h) 5.05 ppm(w/v). 

Thus according to static Fish Test the BCF value for test substance was observed to be 34-143 L/kg at dose concentration 0.1 ppm(w/v) and 38- 235 L/kg at dose concentration 0.01 ppm(w/v) on test organism Cyprinus carpio during 8 weeks period.

These BCF values indicate that the substance is not bioaccumulative in fish and food chain.

Key value for chemical safety assessment

Additional information

Experimental studies and one predicted study of test chemical were reviwed for bioaccumulation endpoint and their results are summarized below.

The first study was experimental study in this study the Bioaccumulation test was conducted for 8 weeks for determination the bioconcentration factor (BCF) of substance on test organism Cyprinus carpio.

The recovery ratio is given- Test water: 1st concentration area: 91.4 %, 2nd concentration area: 84.1 %, Fish: 73.5 %, Vehicle used as Tween 80 with concentrations 1st Concentration area: 1 ppm(w/v), 2nd Concentration area: 0.1 ppm(w/v) respectively.

And Nominal concentrations used in the study as - 1st Concentration area: 0.1 ppm(w/v), 2nd Concentration area : 0.01 ppm(w/v) and Range finding study was carried out on Rice fish (Oryzias latipes) and the 48 hr TLm(48h) 5.05 ppm(w/v). 

Thus according to static Fish Test the BCF value for test substance was observed to be 34-143 L/kg at dose concentration 0.1 ppm(w/v) and 38- 235 L/kg at dose concentration 0.01 ppm(w/v) on test organism Cyprinus carpio during 8 weeks period.

These BCF values indicate that the substance is not bioaccumulative in fish and food chain.

Next study was reviewed from authorative database HSDB, the bioaccumulation factor (BCF) of test chemical in aquatic organisms was estimated to be 3.0 dimensionless using an estimated log Kow of 3.15 and a regression derived equation.This BCF suggests the potential for bioconcentration in aquatic organisms is low.

To support the results of above mentioned studies one prediction was done by using BCFBAF Program (v3.00) model of EPI suite in this the bio concentration factor (BCF) for test chemical was estimated to be 56 L/kg wet-wt which does not exceed the bioconcentration threshold of 2000.Thus it is concluded that test chemical is not expected to bio accumulate in the aquatic environment.

By considering results of all the studies mentioned above it is concluded that BCF value of test chemical is in range of 3.0 to 235 L/kg. On the basis of this range value it is concluded that test chemical is non bioaccumulatie in nature.