4,11-diamino-2-(3-methoxypropyl)-1H-naphth[2,3-f]isoindol-1,3,5,10(2H)-tetrone

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

None

GLP compliance:

yes

Specific details on test material used for the study:

None

Analytical monitoring:

yes

Details on sampling:

Not available

Vehicle:

yes

Details on test solutions:

In each test concentration and controls 20 Daphnia were tested, divided into four groups of five animals each in 50 ml test medium in a glass-beaker (100 ml). The test was performed in an air-conditioned room.

Test organisms (species):

Daphnia magna

Details on test organisms:

The study was performed with young Daphnia magna Straus (6-24 hours old), bred in the laboratories of RCC under standardized conditions. The parental Daphnia were maintained in the same reconstituted water as in the test for at least 24 hours prior to the start of the test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

None

Hardness:

2.5 mmol/L as CaCO3

Test temperature:

18.0 - 19.5 °C

pH:

None

Dissolved oxygen:

None

Salinity:

0.8 mmol/L

Nominal and measured concentrations:

The final nominal concentrations were: 0.04, 0.20, 0.40, 2.0 and 4.0 mg test substance/L, a control without any additions and a solvent control ( 0.1 ml Dimethylformamid per 1 test medium).

Details on test conditions:

None

Reference substance (positive control):

not specified

Key result

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

>= 4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

In the controls no immobility or mortality of the test animals was observed.

Results with reference substance (positive control):

None

Reported statistics and error estimates:

None

Influence of FAT 36152/D on the motility of Daphnia magna:

Nominal Conc. Of test material (mg/l)	No. of Daphnia tested	No. of immobilixzed Daphnia after:		% of immobilized Daphnia after:
		24 h	48 h	24 h	48 h
Control	20	0	0	0	0
Solvent control	20	0	0	0	0
0.04	20	0	0	0	0
0.2	20	0	0	0	0
0.4	20	0	0	0	0
2	20	0	3	0	15
4	20	0	0	0	0

Dissolved oxygen concentrations (mg/l) and pH-values in the test media at the start and end of the acute toxicity test with Daphnia magna:

Nominal Conc. Of test material (mg/l)	Start (0 hour)		End (48 hours)
	Oxygen	pH	Oxygen	pH
Control	9	8	8.5	7.7
Solvent control	9.3	8	8.8	7.5
0.04	*	*	9	7.5
0.2	*	*	9	7.5
0.4	*	*	9	7.5
2	*	*	9	7.5
4	9.1	8	9	7.5

Validity criteria fulfilled:

yes

Conclusions:

The NOEC (highest concentration tested without toxic effect) of FAT 36'152/D was determined to be 4.0 mg/L or higher.

Executive summary:

Short term toxicity of FAT 36152/D to Daphnia magna was determined in a 48-hour static test conducted according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4 April 84. The test was performed in compliance with the Good Laboratory Practice Regulations. The nominal concentrations tested were 0.04, 0.20, 0.40, 2.0 and 4.0 mg test substance/L, a solvent control group and a control without any additions. In the control no immobility or mortality of the test animals was observed.

FAT 36'152/D had no acute toxic effect on Daphnia magna during the test duration of 48 hours up to the highest concentration tested of 4.0 mg test substance/l (as the highest concentration that was possible to dissolve in dimethylformamid p.A. at room temperature and an addition of 100 µL solvent stock solution to 1 L test medium). Therefore, the NOEC (highest concentration tested without toxic effect) of FAT 36152/D was determined to be 4.0 mg/L or higher. The 48 h EC50 could not be specified. The test substance could be detected only in a sample of the analysed test media of the highest concentration tested of 4.0 mg/L, but also in this concentration the recovery rate was low (2.8 % of the nominal value). In samples of the lower test concentrations the test substance was not detectable. Due to the fact that the test substance is not soluble in water, it obviously precipitated and homogeneous samples for the analytical measurements could not be taken. Since the measured concentration in the stock solution amounted to 98.8 % of the nominal value, the reported results are related to the nominal concentrations of the test substance.  

Description of key information

The EC50 was higher than 4 mg/l after 48 hours exposure. 

Additional information

Short term toxicity of FAT 36152/D to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4 April 84. The nominal concentrations tested were 0.04, 0.20, 0.40, 2.0 and 4.0 mg test substance/L, a solvent control group and a control without any additions. In the control no immobility or mortality of the test animals was observed. FAT 36'152/D had no acute toxic effect on Daphnia magna during the test duration of 48 hours up to the highest concentration tested of 4.0 mg test substance/l (as the highest concentration that was possible to dissolve in dimethylformamid p.A. at room temperature and an addition of 100 µLsolvent stock solution to 1 L test medium). Therefore, the NOEC (highest concentration tested without toxic effect) of FAT 36152/D was determined to be 4.0 mg/L or higher.