Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2005
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report Date:
2005

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Version / remarks:
and EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, adapted
Details on inoculum:
- Source of inoculum/activated sludge: collected from a water treatment plant containing effluent from a predominantly domestic origin,
from the water treatment plant of Evreux, France
- Preparation of inoculum for exposure: The inoculum was prepared by initially decanting and sieving sewage sludge. The sludge was then centri-
fuged for 5 minutes, the supernatant was rejected and the pellet was redispersed in the mineral medium.
- Concentration of sludge: 20 mg / l of suspended solids (dry weight) in 3 lires of suspension
- Water filtered: yes
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
10 mg/L
Based on:
other: TOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
TOC removal
Details on study design:
Measurements of CO2 on days 1, 4, 6, 8, 11, 14, 18, 22, 25, 29.
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

Preliminary study:
None.
% Degradation
Parameter:
% degradation (TOC removal)
Value:
77
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
77 % after 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
not readily biodegradable