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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2004
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report Date:
2005

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
Version / remarks:
and EU Method C.3 (Algal Inhibition test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: algae (Scenedesmus subspicatus (new name: Desmodesmus subspicatus))
- Strain: CCAP 276/20
- Source (laboratory): SAMS Research Services Ltd Dunstaffnage Laboratory, Scotland
culture collection Culture Collection of Algae and Protozoa
Cultured at CIT
- Age of inoculum (at test initiation): 10 days
- Method of cultivation: under sterile conditions and maintained at exponential growth rate


ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test): reconstitued water (LC)
- Any deformed or abnormal cells observed: no

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Hardness:
34 ± 17 mg CaCO3 / l
Test temperature:
21 -25 °C not varying by more than ± 2 °C during the test
pH:
7.5 ±0.3 not deviating more than 1.5 unit during the tests
Dissolved oxygen:
Forced aeration was not used during the test, but the cultures were constantly agitated.
Nominal and measured concentrations:
See the table below (any information on materials and methods icl. tables.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
other: EbC50
Effect conc.:
3.88 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
other: ErC50
Effect conc.:
5.18 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
2.22 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
other: growth rate and biomass

Applicant's summary and conclusion

Validity criteria fulfilled:
yes