Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2004
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report Date:
2005

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
and EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus-clone 5
- Source: Centre Technique du Bois et de l'Ameublement, Paris, France Breeder: CIT
- Age at study initiation (mean and range, SD): 6-24 hours
- Method of breeding: from parthenogenically reproducing brood female population
- Feeding during test animals were not fed during the test.


ACCLIMATION
- Acclimation period: from birth until use in the test
- Acclimation conditions (same as test or not): not
- Type and amount of food: Scenedesmus subspicatus, 0.1-0.2 mg of carbon / Daphnia / day

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Test conditions

Hardness:
289 mg CaCO3 / l
Test temperature:
18 - 22 °C not varying by more than 1°C throughout the test
pH:
6 - 9 and not varying by more than 1 unit in the solutions during the test
Dissolved oxygen:
≥ 60 % of the air saturation value
Salinity:
sum of Ca and Mg ions in the solution: 2.5 mmol / l ( molar ratio: Ca/Mg : 4:1, Na/K: 10:1)
Nominal and measured concentrations:
See the table below (any information on materials and methods icl. tables.

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
6.04 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
8.29 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Under our experimental conditions, the 48-hour EC50 in a static test system was 6.04 mg/L for Daphnia magna.