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1-((2-Butyloctyloxymethyl)-2-(3,4-dihydro-isoquinolinium-2-yl)ethyl)sulfate was tested in a carbon dioxide evolution test according to OECD guideline 301B. The test was left running for 28 days. Aniline was chosen as positive control. Biodegradation of the positive control was 99.7% (28th day). The biodegradation of the test item is 26 % (28th day). In this test, the test substance is considered partly or moderately biodegradable, but the test substance is considered not readily biodegradable.