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EC number: 443-520-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-05-14 - 2002-05-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.6800 (Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 2000-04-26
Test material
- Reference substance name:
- -
- EC Number:
- 443-520-7
- EC Name:
- -
- Cas Number:
- 33016-77-2
- Molecular formula:
- C2H4Na4O6P2
- IUPAC Name:
- tetrasodium (1-phosphonatoethenyl)phosphonate
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- - Synonyms: ITC 908, vinylidenephosphonic acid tetrasodium salt (VDPA tetrasodium salt)
- Lot/batch No.of test material: 562CH/646
- Purity: 87.33%
- Storage condition of test material: Room temperature, in the dark
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: A mixed population of activated sewage sludge micro-organisms was obtained on 16 May 2002 from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK which treats predominantly domestic sewage.
- Preparation of inoculum for exposure: The sample was maintained on continuous aeration in the laboratory at a temperature of 21 °C and was used on the day of collection.
- Concentration of sludge: Determination of the suspended solids level of the activated sewage sludge was carried out by filtering a sample (100 ml) of the activated sewage sludge by suction through a piece of pre-weighed GF/A filter paper using a Buchner funnel. The piece of GF/A filter paper was then dried in an oven at approximately 110 °c for 1 hour and allowed to cool before reweighing. The suspended solids was equal to 4.0 g/L prior to use.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 21 °C
- pH:
- 7.9 - 8.1
- Nominal and measured concentrations:
- Range-finding test 1: 1.0, 10, 100 and 1000 mg/L
Range-finding test 2: 2000 mg/L
Definitive test: 32, 100, 320, 1000 and 3200 mg/L - Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The test water used for both the range-finding and definitive tests was laboratory tap water dechlorinated by passage through an activated carbon filter and partly softened giving water with a total hardness of approximately 100 mg/L as CaCO3. After dechlorination and softening the water was then passed through a series of computer controlled plate heat exchangers to achieve the required temperature.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Normal laboratory lighting conditions
TEST CONCENTRATIONS
Range finding study
- Test concentrations: 1) 1.0, 10, 100 and 1000 mg/L; 2) 2000 mg/L
- Results used to determine the conditions for the definitive study: Inhibition of respiration (14%) was observed at 1000 mg/L in the initial range-finding test, howevere, there were no significant effects on respiration at 1.0, 10 and 100 mg/L. Therefore, in order to confirm that the result obtained from the initial range-finding test at the test contration of 1000 mg/L was signification a second range-finding test was conducted at a test concentration of 2000 mg/L. Significant inhibition of respiration was observed at 2000 mg/L in the second range finding test. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol: 3.2, 10 and 32 mg/L
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- In some instances, the initial and final dissolved oxygen concentrations were below those recommended in the test guidelines. This was considered to have had no adverse effect on the results of the study given that in all cases the oxygen consumption rate was determined over the linear portion of the oxygen consumption trace.
- Results with reference substance (positive control):
- 30min EC50: 17 mg/L
3h EC50: 12 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The effect of the test material on the respiration of activated sewage sludge micro-organisms gave a 3-hour EC50 of 1500 mg/L. The NOEC after 3 hours exposure was 320 mg/L
- Executive summary:
The effect of the test material on the respiration of activated sewage sludge micro-organisms was tested according to OECD TG 209 and under GLP conditions. A 3-hour EC50 of 1500 mg/L was determined. The NOEC after 3 hours exposure was 320 mg/L
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