1,3-dichloropropane

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1983

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: Methods for chemical analysis of water and wastes. Washington, DC: U.S. Environmental Protection Agency, Office of Technology Transfer (1974).

Version / remarks:

no further specification

Deviations:

not specified

Principles of method if other than guideline:

reliable data from handbook or collection of data but without specification of method
- Principle of test:
test item is dissolved in freshwater which is used as the medium for exposure of fish
- Parameters analysed / observed:
lethal effects on fish

GLP compliance:

no

Remarks:

Quality maintained by educational and professional experience of experimentators/authors

Test material

Test material information

Constituent 1

Reference substance name:

1,3-dichloropropane

EC Number:

205-531-3

EC Name:

1,3-dichloropropane

Cas Number:

142-28-9

Molecular formula:

C3H6Cl2

IUPAC Name:

1,3-dichloropropane

Test material form:

liquid

Specific details on test material used for the study:

no data available

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

twice a day dead fish were counted and removed from flow through tanks.
Chemical analysis on hexane extract of incubation water

Test solutions

Vehicle:

no

Remarks:

Lake Superior fresh water was the source of the dilution water

Details on test solutions:

Water chemistry methods according to American Public Health Association (APHA (American Public Health Association), American Water Works Association, and Water Pollution Control Federation: Standard methods for the examination of water and wastewater. 15 ed. Washington, DC: American Public Health Assoc. (1980).) and the U.S. Environmental Protection Agency (Methods for chemical analysis of water and wastes. Washington, DC: U.S. Environmental Protection Agency, Office of Technology Transfer (1974)).

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

30-35 d old, reared in diluent water at 25 °C +/- 2 °C;
fed live brine shrimp nauplii in excess until 12 to 24 h before testing
No feeding during exposure

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

45.1 mg/L as CaCO3 (45.0-45.5)

Test temperature:

25.0 +/- 0.7 °C

pH:

6.7 to 7.6

Salinity:

alkalinity 41.8 mg/L as CaCO3 (35.6-43.4)

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 124 - <= 137 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: LC50 = 131 mg/L

Effect concentrations 2

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

>= 124 - <= 137 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: LC50 = 131 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

>= 124 - <= 137 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: LC50 = 131 mg/L

Effect concentrations 4

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

>= 126 - <= 139 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: LD50 = 133 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

LC50 Pimephales promelas (96 hours)
Minimum: 87 mg/l
Maximum: 131 mg/l
Median: 113 mg/l

Executive summary:

LC50 Fish (96 hours) Minimum: 87 mg/l