beta-D-fructofuranosyl alpha-D-glucopyranoside octa(acetate and palmitate and stearate)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 Sep - 21 Oct 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Sucrose tetrastearate triacetate

IUPAC Name:

Sucrose tetrastearate triacetate

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

other: Secondary effluent

Details on inoculum:

- Concentration of sludge: 1 mL/L
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Brazos Valley River Sewer South (date of collection: 16 Sep 2014)

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

2 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 22 °C
- Aeration of dilution water: aerated for 20 min
- Continuous darkness: yes

TEST SYSTEM
- Number of culture flasks/concentration: 10 (BOD bottles)
- Measuring equipment: Oxygen was measured in each duplicate using an YSI Model 5100 dissolved oxygen meter

SAMPLING
- Sampling frequency: Day 0, 7, 14, 21, and 28

CONTROL AND BLANK SYSTEM
- Abiotic sterile control: mineral media
- Toxicity control: 0.6 mg of test substance was added to BOD bottles filled with aniline solution

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

-15.7

Sampling time:

7 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

-8.8

Sampling time:

14 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

14.7

Sampling time:

21 d

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

17.1

Sampling time:

28 d

Details on results:

The toxicity control showed sufficient O2 depleteion, indicating that the test item and reference material are not toxic to the inoculum.

BOD5 / COD results

Results with reference substance:

The reference substance, aniline, was 92.2% biodegraded by Day 7, 108.5% by Day 14 and 117.2% biodegraded by Day 21.

Any other information on results incl. tables

Table 1: Dissolved oxygen measurements

	Dissolved oxygen [mg/L]
Test group	Day 0	Day 7	Day 14	Day 21	Day 28
Blank	8.27	7.87	7.86	8.00	7.89
	8.27	8.01	7.88	8.00	7.89
Reference substance	8.35	3.63	2.72	2.57	2.47
	8.39	3.58	2.79	2.36	2.22
Test item	8.32	8.08	7.92	7.96	7.73
	8.27	8.04	7.98	7.91	7.89
Toxicity control	8.38	3.65	2.75	-	-
	8.39	3.47	2.58	-	-

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test substance biodegraded only 17.1% in 28 days. Thus, the test substance is not readily biodegradable according to the OECD criteria.

Executive summary:

The biodegradation of the test substance was determined using an OECD Guideline 301D study. The reference substance, aniline, was 92.2% biodegraded by Day 7, and 117.2% biodegraded by Day 21. Thus the test was valid. The test substance biodegraded only 17.1% in 28 days. The test substance is therefore not readily biodegradable.