Registration Dossier
Registration Dossier
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EC number: 280-349-5 | CAS number: 83261-15-8
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP guideline study according to the German National Standard (DIN).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- GLP compliance:
- no
Test material
- Reference substance name:
- Isobutyl-2-chloropropionate
- IUPAC Name:
- Isobutyl-2-chloropropionate
- Reference substance name:
- Isobutyl (S)-2-chloropropionate
- EC Number:
- 280-349-5
- EC Name:
- Isobutyl (S)-2-chloropropionate
- Cas Number:
- 83261-15-8
- Molecular formula:
- C7H13ClO2
- IUPAC Name:
- isobutyl (S)-2-chloropropanoate
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): Stock solution: 100 mg/l
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Initial biomass concentration: 10 TE/F
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 17 h
Test conditions
- pH:
- 4.9 - 7.3
- Nominal and measured concentrations:
- Nominal: 156, 313, 625, 1250, 2500 and 5000 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 300 ml glass Erlenmeyer
- Aeration: none
- No. of organisms per vessel: 10 TE/F
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK-Medium accodring to DiN 38412 part 8 (draft)
- Culture medium different from test medium: no
EFFECT PARAMETERS MEASURED: growth inhibition after 7h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Test concentrations: 156, 313, 625, 1250, 2500 and 5000 mg/l
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 17 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2 795.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 17 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4 217.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
