Registration Dossier
Registration Dossier
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EC number: 280-349-5 | CAS number: 83261-15-8
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP study according to the German National Standard (DIN).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412 part 9
- GLP compliance:
- no
Test material
- Reference substance name:
- Isobutyl (S)-2-chloropropionate
- EC Number:
- 280-349-5
- EC Name:
- Isobutyl (S)-2-chloropropionate
- Cas Number:
- 83261-15-8
- Molecular formula:
- C7H13ClO2
- IUPAC Name:
- isobutyl (S)-2-chloropropanoate
- Reference substance name:
- Isobutyl-2-Chloropropionate
- IUPAC Name:
- Isobutyl-2-Chloropropionate
- Details on test material:
- - Name of test material (as cited in study report): L-Chlorpropionsaeureisobutylester
- no further details on the test substance characteristics are given
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- REPARATION AND APPLICATION OF TEST SOLUTION
- Method: Concentration of the stock solution was 1000 mg/l.
- Differential loading:
- Controls: Negative control and solvent (Tween 80) control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): dispersant Tween 80
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): stock solution: 20 mg/l
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: CHODAT SAG 86.81
- Source (laboratory, culture collection): Supplier: Sammlung für Algenkulturen der Universität Göttingen
- Age of inoculum (at test initiation): The test algae are taken from a 3 days-old continously growing preculture
- Method of cultivation: The test strain is kept in liquid culture in the laboratory.
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions: same as test medium
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 19 - 21 °C
- pH:
- 8.2 - 8.5
- Nominal and measured concentrations:
- Nominal: 15.6, 31.25, 62.5, 125, 250 and 500 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open / closed
- Material, size, headspace, fill volume: Erlenmeyer flasks (nominal 250 ml), plugged with gas permeable siliconesponge caps, 100 ml test volume
- Initial cells density: 10000 cells/ml
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412/9
OTHER TEST CONDITIONS
- Photoperiod: continous
- Light intensity and quality: white light (about 60 - 120 microEinstein/m*m*s), wave length 400 - 700 nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: in vivo chlorophyll-a-fluorescence at 685 nm wavelength (as measure for cell number) after 0, 24, 48, and 72 hours
- pH values: after 0 and 72 hours
TEST CONCENTRATIONS
31.3, 62.5, 125, 250 and 500 mg/L. A control was tested in parallel.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 159 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- 1 160 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 7.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 49 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- 323 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
