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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
The test substance (2 g) was dispersed in dilution medium (2 l) in a volumetric flask. The contents of the flask were shaken vigorously and treated
with ultrasound for 20 minutes and then used directly at 1000 mg/l or diluted to provide the test media at the lower concentrations

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Stock cultures of Daphnia magna were maintained in glass vessels containing approximately 0.8 litres of Elendt M4 culture medium in a temperature
controlled laboratory at nominally 20 ± 2°C. A photoperiod of 16 hours light : 8 hours dark was maintained, with periods of subdued lighting at the
beginning and end of each light phase. The culture medium was renewed three times each week.
Cultures were fed daily with a suspension of the unicellular green alga, Pseudokirchneriella subcapitata, to provide nominally 0.1 to 0.2 mg carbon
per daphnid, per day, except during the initial three days when a slightly lower concentration was given. Culture conditions ensure that the stock
animals reproduce by parthenogenesis

Study design

Test type:
static
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20.4 to 21.2 °C
pH:
7.82 to 8.12
Dissolved oxygen:
98 to 103%
Nominal and measured concentrations:
nominal 42.7, 93.9, 207, 455 and 1000 mg/L
mean measured: 39.8, 93.6, 229, 471 and 1111 mg/l.

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 93.6 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: N/A
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
93.6 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 81.1 and 108 mg/L
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
221 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
39.8 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Conclusions:
The 48-hour EC50 of VRT-126017 dcha for the immobilisation of Daphnia magna was 93.6 mg/l (95% confidence limits of 81.1 and 108 mg/l).
The ‘no-observed effect concentration’ of VRT-126017 dcha with Daphnia magna was 39.8 mg/l.