Bis(cyclohexyl)ammonium(2S)-2-{[(benzyloxy)carbonyl]amino}-3,3-dimethylbutanoate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

478-250-9

EC Name:

-

Cas Number:

62965-37-1

Molecular formula:

C26H42N2O4

IUPAC Name:

(2S)-2-{[(benzyloxy)carbonyl]amino}-3,3-dimethylbutanoic acid; N-cyclohexylcyclohexanamine

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

The test substance (2 g) was dispersed in dilution medium (2 l) in a volumetric flask. The contents of the flask were shaken vigorously and treated
with ultrasound for 20 minutes and then used directly at 1000 mg/l or diluted to provide the test media at the lower concentrations

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Stock cultures of Daphnia magna were maintained in glass vessels containing approximately 0.8 litres of Elendt M4 culture medium in a temperature
controlled laboratory at nominally 20 ± 2°C. A photoperiod of 16 hours light : 8 hours dark was maintained, with periods of subdued lighting at the
beginning and end of each light phase. The culture medium was renewed three times each week.
Cultures were fed daily with a suspension of the unicellular green alga, Pseudokirchneriella subcapitata, to provide nominally 0.1 to 0.2 mg carbon
per daphnid, per day, except during the initial three days when a slightly lower concentration was given. Culture conditions ensure that the stock
animals reproduce by parthenogenesis

Study design

Test type:

static

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20.4 to 21.2 °C

pH:

7.82 to 8.12

Dissolved oxygen:

98 to 103%

Nominal and measured concentrations:

nominal 42.7, 93.9, 207, 455 and 1000 mg/L
mean measured: 39.8, 93.6, 229, 471 and 1111 mg/l.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 93.6 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: N/A

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

93.6 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 81.1 and 108 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

221 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

39.8 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Conclusions:

The 48-hour EC50 of VRT-126017 dcha for the immobilisation of Daphnia magna was 93.6 mg/l (95% confidence limits of 81.1 and 108 mg/l).
The ‘no-observed effect concentration’ of VRT-126017 dcha with Daphnia magna was 39.8 mg/l.