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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
The test concentrations of VRT-126017 dcha were measured using an LC-MS method of analysis. Four, mid-vessel samples (10 ml) of media were
taken from the control and test media at 0 and 72 hours (fresh media) and at 24 and 96 hours (expired media) for analysis.

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout (Oncorhynchus mykiss)
- Length at study initiation: 6.88 cm
- Feeding during test: no

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: temperature 13.9-14.3°C, pH 7.65-7-98, dissolved oxygen 92-96%, total hardness 132-170 mg/L as CaCO3
- Type and amount of food: commercial fish food (TROUW (UK) Ltd; Nutra Trout Fry 02) an amount equivalent to between 0.75 and 1.0% of the total wet-weight of fish
- Feeding frequency: daily
- Health during acclimation (any mortality observed): No medication was given during the holding period, and mortalities were recorded as <1.0%
in the 14 days before the definitive test.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
152-164 mg/L as CaCO3
Test temperature:
14.2-15.7°C
pH:
7.67-8.38
Dissolved oxygen:
91-108%
Nominal and measured concentrations:
nominal 4.27, 9.39, 20.7, 45.5 and 100 mg/L
measured 4.16, 10.1, 20.9, 45.4 and 88.5 mg/L
Details on test conditions:
Groups of seven juvenile fish were exposed to VRT-126017 dcha, dissolved in water at nominal concentrations of 4.27, 9.39, 20.7, 45.5 and
100 mg/l. The test media were prepared by direct addition of the test substance to water; to aid dissolution, ultrasound treatment (30 minutes) was
employed.

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
58.9 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
58.9 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
58.9 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
58.9 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
88.5 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
4.16 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion

Conclusions:
The 96-hour LC50 value for VRT-126017 dcha with rainbow trout was 58.9 mg/l, with 95% confidence limits of 39.6 and 107 mg/l.
The “no observed effect concentration” was considered to be 4.16 mg/l.