Propan-2-ol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study without detailed documentation.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

; 4 fish per concentration instead of 7 per concentration, individual test concentrations were not reported

Qualifier:

according to guideline

Guideline:

other: U.S. Environmental Protection Agency Committee on Methods for Toxicity Tests with Aquatic Organisms (1975)

Deviations:

no

Principles of method if other than guideline:

The 96 hour LC50 method is described by the US Environmental Protection Agency Committee on Methods for Toxicity Tests with Aquatic Organisms 1975.

GLP compliance:

no

Remarks:

Study pre-dates ecotoxicity GLP requirements

Analytical monitoring:

yes

Details on sampling:

Not reported.

Vehicle:

no

Details on test solutions:

A primary stock solution was prepared by dissolving the test substance directly in dilution water.

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: Fathead Minnow
- Source: U.S. Environmental Protection Agency, Environmental Research Laboratory-Duluth culture, Duluth, MN 55804
- Age at study initiation (mean and range, SD): 30-day old
- Weight at study initiation (mean and range, SD): 0.12 g
- Feeding during test: Fish were not fed during the test

ACCLIMATION: Information concerning acclimation was not reported.

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

56.3 mg/L as Calcium Carbonate

Test temperature:

25 ± 1°C

pH:

7.5

Dissolved oxygen:

Greater than 60% of saturation

Salinity:

Freshwater

Nominal and measured concentrations:

Not reported.

Details on test conditions:

TEST SYSTEM
- Type of flow-through (e.g. peristaltic or proportional diluter): Proportional diluters
- Renewal rate of test solution (frequency/flow rate): Not reported
- No. of organisms per vessel: 2
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Lake Superior water
- Total organic carbon: Not reported
- Particulate matter: Not reported
- Metals: Not reported
- Pesticides: Not reported
- Chlorine: Not reported
- Alkalinity: 42.2 mg/L Calcium Carbonate
- Ca/mg ratio: Not reported
- Conductivity: Not reported
- Intervals of water quality measurement: Not reported

OTHER TEST CONDITIONS
- Photoperiod: Not reported
- Light intensity: Not reported

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Deaths were recorded after 1, 3, 6, 12, 24, 48, 72, and 96 hours.

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

10 000 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Toxicity test #1.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

9 640 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Toxicity test #2.

Details on results:

- Mortality of control: Not reported.

Results with reference substance (positive control):

no data

Reported statistics and error estimates:

Not reported

Validity criteria fulfilled:

not specified

Remarks:

insufficient documentation for assessment

Description of key information

The substance was not acutely toxic to freshwater fish when tested according to the methods defined by the US Environmental Protection Agency Committee on Methods for Toxicity Tests with Aquatic Organisms, the substance was determined to possess a 96-hour LC50 in freshwater fish ranging between 9640 and 10000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

9 640 mg/L

Additional information

Three studies addressing short-term toxicity to fish are presented in the dossier. 

Short-term toxicity to fish.001 – 1983 – key: Acute toxicity to the freshwater fish Pimephales promelas (Fathead minnow) was assessed according to the methods defined by the US Environmental Protection Agency Committee on Methods for Toxicity Tests with Aquatic Organisms not according to GLP (pre-dates GLP requirements). The study was conducted under flow through conditions under the use of proportional diluters with 4 fish per concentration (individual test concentrations not reported) for 96 hours. Temperature was maintained at 25 ± 1°C and the concentrations were measured in each tank throughout the test. The percentage of dead fish was recorded at 1, 3, 6, 12, 24, 48, 72 and 96 hours. The following validity criteria were met: dissolved oxygen concentrations remained above 60% of the air saturation value at the end of the test; and the pH of the control and of the test solution did not vary by more than one unit during the course of the test. Mortality of the controls was not reported and although concentrations were measured throughout the test, individual nominal and measured concentrations were not reported. Two separate tests resulted in 96 hour LC50s of 10,000 mg/L and 9640 mg/L.

Short-term toxicity to fish.003 – Golden orf - 1978: Acute toxicity to the freshwater fish Leuciscus idus melanotus (Golden orf) was assessed according to Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung. L 15: Fischtest (Bestimmung der Wirkung von Wasserinhaltsstoffen auf Fische) not according GLP (pre-dates GLP). The fish were exposed to the substance under static conditions for 48 hours. The 48 – hour LC50 was determined to range between 8970 and 9280 mg/L. This lower toxicity over a shorter exposure period is in keeping with the result of the key study.

Short-term toxicity to fish.002 - Goldfish - 1973: Acute toxicity to the freshwater fish Carassius auratus (Goldfish) was assessed according to OECD guideline 203 (Fish, acute toxicity test) not according GLP (pre-dates GLP). The fish were exposed to the substance under static conditions for 24 hours.  The median tolerance limit (TLm: the concentration at which 50% animals survive) at 24 hours was reported to be >5000 mg/L. Detailed information was not provided in the report however, the results are in line with the key study. Therefore, result from a non reliable study however supports the results from the 2 reliable studies presented above.