Acetaldehyde

Long-term toxicity to fish

Administrative data

Endpoint:

adult fish: sub(lethal) effects

Type of information:

experimental study

Adequacy of study:

key study

Study period:

not stated

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to Guideline study with acceptable restrictions. In this test, the LC50 was determined and not the NOEC as required in OECD 204

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1988

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)

Deviations:

yes

Remarks:

LC50 was determined instead of a NOEC

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Acetaldehyde

EC Number:

200-836-8

EC Name:

Acetaldehyde

Cas Number:

75-07-0

Molecular formula:

C2H4O

IUPAC Name:

acetaldehyde

Details on test material:

- Name of test material: Ethanal

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

- Chemical name of vehicle: acetone
- Concentration of vehicle in test medium: control fish were exposed to 72 µl acetone

Test organisms

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Common name: Poecilia reticulata
- Source: laboratory reared
- Age at study initiation: 2-3 month


ACCLIMATION
- Acclimation period: at least 12 days prior to experiment
- Acclimation conditions: same as test
- Type and amount of food: fish were fed a commercial food (TetraPhyll, Tetra Werke, Germany)
- Feeding frequency: daily

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

21 - 23°C

pH:

pH was determined four times immediately before, and immediately after renewal of the test solution for to concentrations. Results are not given in the study

Dissolved oxygen:

Oxygen content was determined four times immediately before, and immediately after renewal of the test solution for to concentrations. Results are not given in the study

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: 1.5 l glass vessels which contained 1.4 l of the test solution
- Renewal rate of test solution (frequency/flow rate): test solution was renewed daily
- No. of organisms per vessel: 10


OTHER TEST CONDITIONS
- Photoperiod: 12 hours light/12 hours dark


EFFECT PARAMETERS MEASURED: mortality


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 d

Dose descriptor:

other: log LC50

Effect conc.:

2.9 µmol/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: log LC50 is given here which is originally stated in the report

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

794.3 µmol/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: converted value

Effect concentrations 3

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

ca. 35 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: converted value, based on a molar mass of 44.05 g/mol

Reported statistics and error estimates:

LC50 was calulates by logit transformation

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

After an exposure period of 14 day, the LC50 was determined to be 35 mg/l based on nominal values. Although a NOEC and not a LC50 is required according to the guideline, study is suitable as supporting information in the chemical safety assessment.

Executive summary:

The prolonged toxicity of acetaldehyde was tested semi-static test over a period of 14 days similar to OECD 204. Guppies were used as test organisms. Fish were exposed to five test concentration using a spacing factor of 1.8. As endpoint, mortality was determined and LC50 was calculated by logit transformation. Analytical measurements could not be conducted because no reliable recovery factors could be determined and the results were therefore based on nominal values. The log LC50 which is stated in the report was calculated to be 2.9 µmol/l which corresponds to 35 mg/l based on nominal values.