Registration Dossier
Registration Dossier
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EC number: 246-278-9 | CAS number: 24468-13-1
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Parent compound: acutely toxic to fish.
Hydrolysis product 2-ethylhexanol: acutely harmful to fish.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Remarks:
- Parent compound 2-ethylhexyl chloroformate
- Effect concentration:
- 3.16 mg/L
Fresh water fish
- Dose descriptor:
- LC50
- Remarks:
- Hydrolysis product 2-ethylhexanol (CAS 104-76-7)
- Effect concentration:
- 28.2 mg/L
Additional information
The acute toxicity on fish was assessed in two experimental studies with Danio rerio (Hoechst AG,1988) and Leuciscus idus (BASF AG, 1988). The tests were performed with the parent compound 2-ethylhexyl chloroformate, which is expected to hydrolyse rapidly in contact with water (DT50 at 25 °C and pH 7 = 30.2 min, Safepharm, 2003) to form 2-ethylhexanol (CAS 104-76-7), hydrogen chloride (HCl, CAS 7647-01-0) and CO2. Both studies were performed without chemical analysis therefore observed toxic effects may be based on the test substance itself, its hydrolysis product or the shift in pH due to the production of hydrochloric acid at hydrolysis of the test substance.
However, an influence of the pH of the test water on fish toxicity is unlikely because at all relevant test item concentrations the pH was in the range of the control.
Furthermore, the toxicity of the hydrolysis product 2-ethylhexanol (CAS 104-76-7) is lower compared to the results of the studies using 2-ethylhexyl chloroformate. Therefore, although the half-life of 2-ethylhexyl chloroformate in water is short (DT50 30 min), the observed toxicity mentioned above (Hoechst AG, 1988; BASF AG, 1988) appears to be related to the high reactivity of unhydrolyzed 2-ethylhexyl chloroformate at test start. The higher toxicity may be explained by reactions with nucleophiles (such as -NH2, -SH and –OH) on biological macromolecules.
The acute toxicity of the hydrolysis product 2-ethylhexanol on fish was tested in a 96-hour flow-through study (P. promelas, LC50 = 28.2 mg/L, Geiger et al., 1985). This test was performed with chemical analysis revealing a recovery rate of 102.6 % +- 3.2 % of the test item 2-ethylhexanol.
Summary
Species | Results | Remarks | Reference |
Danio rerio | LC50(96 h) = 4.18 mg/L (nominal), static, OECD 203 | reliability: 2, key study | Hoechst AG, 1988 |
Leuciscus idus | LC50(96 h) = 3.16 mg/L (nominal), static, DIN 38412, part 15 | reliability: 2, key study | BASF AG, 1988 |
Pimephales promelas | LC50(96 h) = 28.2 mg/L (nominal); flow-through, with analytical monitoring, OECD 203 | Hydrolysis product 2- ethylhexanol; reliability: 2, key study | Geiger et al., 1985 |
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