2-nitrotoluene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with restrictions

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

1975

Report date:

1975

Reference 2

Reference Type:

secondary source

Title:

European Union Risk Assessment Report - 2-Nitrotoluene

Author:

European Commission - European Chemicals Bureau

Year:

2008

Bibliographic source:

Office for Official Publications of the European Communities

Materials and methods

Principles of method if other than guideline:

Male rats were exposed to saturated vapour of 2-nitrotoluene at a concentration of 190.8 ppm (1.086 mg/L) for 8 h.

GLP compliance:

no

Remarks:

GLP was not mandatory at the time of the study

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-nitrotoluene

EC Number:

201-853-3

EC Name:

2-nitrotoluene

Cas Number:

88-72-2

Molecular formula:

C7H7NO2

IUPAC Name:

1-methyl-2-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): o-nitrotoluene
- Analytical purity: no data

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS

- Weight at study initiation: 139-180g
- Diet (e.g. ad libitum): Altromin 1324 (Altrogge, Lage/Lippe, Germany) ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: ad libitum


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 24°C

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: air

Details on inhalation exposure:

The animals were exposed to mixture of gas and air in a glass container of 39 liters.
The test substance was filled into a gas washing bottle with fritted glass and weighed before and after the experiment. Through an air calibrated rotameter a stream of air of 400 liters per hour was conducted through the gas washing bottle. The amount of substance used by the air flow in 8 hours was 3420 mg
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus:
- Exposure chamber volume:
- Method of holding animals in test chamber:
- Source and rate of air:
- Method of conditioning air:
- System of generating particulates/aerosols:
- Method of particle size determination:
- Treatment of exhaust air:
- Temperature, humidity, pressure in air chamber:

TEST ATMOSPHERE
- Brief description of analytical method used:
- Samples taken from breathing zone: yes/no

VEHICLE
- Composition of vehicle (if applicable):
- Concentration of test material in vehicle (if applicable):
- Justification of choice of vehicle:
- Lot/batch no. (if required):
- Purity:

TEST ATMOSPHERE (if not tabulated)
- Particle size distribution:
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.):

CLASS METHOD (if applicable)
- Rationale for the selection of the starting concentration:

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

ca. 8 h

Concentrations:

190.8 ppm (1.086 mg/L) for 8 h

No. of animals per sex per dose:

6

Control animals:

other: not applicable

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: the animals were weighed daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathology

Statistics:

no data

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LC50

Effect level:

> 1.086 mg/L air

Exp. duration:

8 h

Remarks on result:

other: 95% CL not reported

Any other information on results incl. tables

There were not mortalities, toxicity and gross lesions within 14-day observation period.

Applicant's summary and conclusion

Interpretation of results:

other: no classification is necessary

Remarks:

Criteria used for interpretation of results: EU

Executive summary:

Hollander (1975):

 

SPF Wistar male rats (6 animals) were exposed to saturated vapour of 2-nitrotoluene, i.e. at a concentration of 190.8 ppm (1.086 mg/L) for 8 h. There were not mortalities, toxicity and gross lesions within 14-day observation period. Therefore, according to EU criteria, no classification is necessary.