Reaction products of alkenes, C10-C14-(branched and linear), C12-rich, with hydrogen and carbon monoxide

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Alcohols, C11-14-iso-, C13-rich

EC Number:

271-235-6

EC Name:

Alcohols, C11-14-iso-, C13-rich

Cas Number:

68526-86-3

Molecular formula:

Unspecified

IUPAC Name:

Alcohols, C11-14-iso-, C13-rich

Details on test material:

- Name of test material (as cited in study report): Tridecanol A
- Physical state: liquid/colourless-clear
- Analytical purity: 99.95%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 3.13; 12.5 and 100 mg/L after 0 h and 72 h analyzing the uninoculated replicates

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solution was prepared for each concentration. The stock solutions were stirred in demineralised water for about 20 h at approximately 20 ± 2 °C. Undissolved test substance was removed by centrifugation for ca. 20 min at ca. 17700 G.
- Differential loading: yes

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: green algae
- Strain: SAG 86.81
- Source: Algae collection Goettingen

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Nominal and measured concentrations:

Nominal test concentrations: control, 3.13, 6.25, 12.5, 25, 50, 100 mg/L
Measured test concentrations (0 h): < 0.1, 2.8, 3.8, 5.6 mg/L
Measured test concentrations (72 h): < 0.1, 0.2, 0.2, 1.3 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: Erlenmeyer flasks plugged with permeable siliconsponge caps, 250 ml, 100 ml
- Initial cells density: 10000 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Test medium: according to EEC Directive 92/69/EEC, Annex V, Part C .3
- Intervals of water quality measurement: Temperature continuously during the whole test period in the climate chamber, pH after 0 h and 72 h in an additional uninoculated replicate and after 72 h in the inoculated replicate No. 1 of each concentration.

OTHER TEST CONDITIONS
- Photoperiod: permanent illumination
- Light intensity and quality: artificial light, type universal white (OSRAM L 25), about 60 - 120 µE/(m2*s) at a wave length of 400 - 700 nm

EFFECT PARAMETERS MEASURED: Biomass and growth rate after 24, 48, 72 h
- Determination of cell concentrations: after 72 h in a counting chamber (Neubauer improved) in replicate No. 2 of the inoculated control
- Chlorophyll measurement: in vivo chlorophyll-a-fluorescence (pulsed excitation with light flashes having a wavelength of 435 nm) after 0, 24, 48 and 72 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

2.02 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

3.15 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

> 3.45 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

< 1.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 6

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

2.27 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 7

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

3.36 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 8

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

< 1.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

biomass

Details on results:

- Validity criteria: Cell multiplication in untreated controls after 72 h was 111 fold

Reported statistics and error estimates:

The EC values are calculated (linear regression analysis) from the concentration-response relationship.

Any other information on results incl. tables

Inhibition of algal biomass and growth rate after 72 h:
          Biomass          Growth rate
mg/L    relative  %        relative %
0       2480      -        0.065    -
100     182      92.7      0.027   58.2  
50      289      88.4      0.034   47.9  
25      556      77.6      0.040   39.0  
12.5    1553     37.4      0.059    9.2  
6.25    1518     38.8      0.059    9.7  
3.13    2015     18.8      0.062    3.8  


The respective results on basis of nominal concentrations:
Effect on biomass (72 h):
EbC10 < 3.13 mg/L 
EbC50 = 15.5 mg/L
EbC90 = 65.1 mg/L
NOEbC < 3.13 mg/L

Effect on growth rate (72 h):
ErC10 = 12.7 mg/L
ErC50 = 57.7 mg/L 
ErC90 > 100 mg/L
NOErC = 12.5 mg/L