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EC number: 701-005-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- (only four strains tested, 2-aminoanthracene was the sole indicator of the efficacy of the S9-mix)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Alcohols, C13-15-branched and linear
- EC Number:
- 287-625-4
- EC Name:
- Alcohols, C13-15-branched and linear
- Cas Number:
- 85566-16-1
- Molecular formula:
- C13 H27 OH - C15 H31 OH
- IUPAC Name:
- Alcohols C13-C15 branched and linear, reaction products of C12-C14 olefines
- Details on test material:
- - Name of test material (as cited in study report): C13 - C15 - Alkohol
- Physical state: colorless liquid
- Analytical purity: 99.3% alcohols (0.5% low boiling substances, 0.2% high boiling substances)
- Composition of test material, percentage of components: 29.5% i-C13-alcohol, 33% n-C13-alcohol, 19% i-C15-alcohol, 17.8% n-C15-alcohol
- Lot/batch No.: from continuous production
- Sustance number: 88/577
- Storage: room temperature
Constituent 1
Method
- Target gene:
- his-gene
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix of Aroclor 1254 induced rat livers (Sprague-Dawley)
- Test concentrations with justification for top dose:
- 20 - 5000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Complete solubility of the test substance in DMSO.
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: see Details on test system and conditions for details
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation) and preincubation
Standard plate test:
The experimental procedure is based on the method of Ames et al ., 1975
Test tubes containing 2 ml portions of soft agar which consists of 100 ml agar (0.6% agar + 0.6% NaCl) and 10 ml amino acid solution (minimal amino acid solution for the determination of mutants: 0.5 mM histidine + 0.5 mM biotin) are kept in a water bath at 45°C, and the remaining components are added in the following order:
0.1 ml test solution
0.1 ml bacterial suspension
0.5 ml S-9 mix (in tests with metabolic activation)
or
0.5 ml phosphate buffer (in tests without metabolic activation)
After mixing, the samples are poured onto Vogel-Bonner agar plates (minimal glucose agar plates) within approx. 30 seconds.
Preincubation test:
The experimental procedure is based on the method described by Yahagi et al. (1977) and Matsushima et al. (1980).
0.1 ml test solution, 0.1 ml bacterial suspension and 0.5 ml S-9 mix are incubated at 37°C for the duration of 20 minutes. Subsequently, 2 ml of soft agar is added and, after mixing, the samples are poured onto the Vogel-Bonner agar plates within approx. 30 seconds.
Composition of the minimal glucose agar:
980 ml aqua dest.
20 ml Vogel-Bonner E medium
15 g Difco bacto agar
20 g D-glucose, monohydrate
After incubation at 37°C for 48 hours in the dark, the bacterial colonies (his+ revertants) are counted.
Positive controls:
The following positive control substances are used to check the mutability of the bacteria and the activity of the S-9 mix:
with S-9 mix:
10 µg 2-aminoanthracene (dissolved in DMSO) for the strains TA 100, TA 98, TA 1537 and TA 1535
without S-9 mix:
5 µg N-methyl-N-nitro-N-nitrosoguanidine (MNNG) (dissolved in DMSO) for the strains TA 100 and TA 1535,
10 µg 4-nitro-o-phenylendiamine (dissolved in DMSO) for the strain TA 98,
100 µg 9-aminoacridine chloride monohydrate (dissolved in DMSO) for the strain TA 1537
NUMBER OF REPLICATIONS: triplicate - Evaluation criteria:
- In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control);
- dose-response relationship;
- reproducibility of the results;
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Standard plate test (20 - 5000 µg/plate) | ||||||
Strain | Metabolic activation system | mean his+/trp+revertant colonies (negative control) | maximum revertant factor (conc. (µg/plate)) | dose dependency | Assessment | maximum revertant factor (positive control) |
TA 98 | no | 23 | 1.0 (20/500/2500/5000) | no | negative | 29 (NPD) |
yes | 34 | 1.1 (20/100) | no | negative | 34.7 (2-AA) | |
TA 100 | no | 114 | 0.9 (100) | no | negative | 14.8 (MNNG) |
yes | 103 | 1.3 (100) | no | negative | 17.2 (2-AA) | |
TA 1537 | no | 9 | 1.3 (20) | no | negative | 47 (AAC) |
yes | 12 | 0.9 (20/100/500) | no | negative | 11.7 (2-AA) | |
TA 1535 | no | 15 | 1.0 (20) | no | negative | 128.4 (MNNG) |
yes | 15 | 1.2 (2500) | no | negative | 10.5 (2-AA) | |
Preincubation test (20 - 5000 µg/plate) | ||||||
Strain | Metabolic activation system | mean his+/trp+revertant colonies (negative control) | maximum revertant factor (conc. (µg/plate)) | dose dependency | Assessment | maximum revertant factor (positive control) |
TA 98 | no | 24 | 1.1 (500) | no | negative | 39.9 (NPD) |
yes | 35 | 1.2 (500) | no | negative | 26.5 (2-AA) | |
TA 100 | no | 110 | 1.1 (500) | no | negative | 10.6 (MNNG) |
yes | 125 | 1.1 (20) | no | negative | 9.5 (2-AA) | |
TA 1537 | no | 8 | 1.2 (100) | no | negative | 43.9 (AAC) |
yes | 12 | 0.8 (20/100/2500) | no | negative | 9.3 (2-AA) | |
TA 1535 | no | 19 | 1.2 (5000) | no | negative | 59 (MNNG) |
yes | 18 | 1.0 (20/5000) | no | negative | 7.1 (2-AA) | |
2-AA = 2-aminoanthracene | ||||||
NPD = N-nitro-o-phenylendiamine | ||||||
MNNG = N-methyl-N-nitro-N-nitrosoguanidine | ||||||
AAC = 9-aminoacridine chloride monohydrate |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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