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EC number: 428-100-3 | CAS number: 94239-04-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- The oxygen depletion in the blank after 28 days was 1.6 mg O2/L. This is just outside the value of 1.5 mgO2/L given in the OECD guideline. This deviation of 0.1 mgO2/L is not considered to have affected the validity of the study.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Substance ID: F6TF
Lot #: AH0148
Purity: 100% - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Secondary effluent was obtained from a laboratory scale rolling tube reactor, which treats predominantly domestic sewage. This was added to the test medium at a level of 1 mL/L.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 35.6 mg/L
- Based on:
- other: nominal concentration (measured 22 mg/L)
- Initial conc.:
- 17.8 mg/L
- Based on:
- other: nominal concentration (measured 11 mg/L)
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- The test medium was made up according to the OECD guideline and contained the following nutrients per litre of deionised water: 8.5 mg of KH2PO4, 21.75 mg of K2HPO4, 33.4 mg of Na2HPO4.2H2O, 0.5 mg of NH4Cl, 22.5 mg of MgSO4.7H2O, 54.3 mg of CaCl2.6H20, 0.20 mg of FeCl3.6H2O and 0.40 mg of EDTA (disodium salt).
The measurement of oxygen uptake was carried out in completely filled and closed glass reagent bottles with ground glass stoppers, the volume of which has been determined as 281 ± 2 ml at 20 ± 2 ºC. The bottles were inoculated with micro-organisms from a mixed population and incubated in the dark at 20 ± 1 ºC. The dissolved oxygen concentration in sacrificial test and control bottles was measured at intervals during the test using a YSI oxygen electrode.
Prior to filling the bottles, the prepared mineral medium and inoculurn was aerated for at least two hours and then left to stand for 30 minutes. On Day 0 of the study 32 control blank bottles were set up containing mineral medium and inoculum only. There were 25 reference compound bottles containing inoculated medium and sodium benzoate. And two sets of 17 test bottles, each containing inoculated medium and 5 or 10 mg of test substance, giving nominal test concentrations of 17.8 and 35.6 mg/L, respectively.
Oxygen uptake was recorded at 5 day intervals during the 28 day experimental period (Day 25 analysis replaced by Day 28). The readings obtained were corrected for the O2 demand of the blank and the biodegradation was calculated as a percentage of the theoretical oxygen demand for the substance under test. - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- The substance was not suitable for COD determination by standard methods. The substance was known to be volatile and during the standard COD determination the test compound would be driven off during the heating part of the analysis. Therefore the biochemical oxygen demand (BOD) values have been compared to the theoretical oxygen demand (ThOD) to allow the calculation of % degradation.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- < 5.9
- Sampling time:
- 28 d
- Remarks on result:
- other: nominal concentration 35.6 mg/L (measured 22 mg/L)
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- < 11
- Sampling time:
- 28 d
- Remarks on result:
- other: nominal concentration 17.8 mg/L (measured 11 mg/L)
- Details on results:
- At Day 0 the measured concentrations of the test substance in the nominal 17.8 and 35.6 mg/L test solutions were both 62% of nominal. At Day 28 the measured concentrations were 62 and 65% of nominal, indicating that the test substance was still present. This also confirms the BOD results that the test substance is not readily biodegradable.
- Parameter:
- ThOD
- Value:
- 969.7 mg O2/g test mat.
- Results with reference substance:
- Sodium benzoate attained a maximum level of biodegradation (based on the B0D:COD ratio) of 109% at day 20. Sodium benzoate achieved 63% at day 10, within the 14 day window allowed for a closed bottle test. Indicating that the activated sludge contained viable organisms.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test substance is not readily biodegradable. Degradation after 28 days was < 11% at 11 mg/L and < 5.9% at 22 mg/L.
- Executive summary:
A closed bottle biodegradation test was conducted with two concentrations of the test substance according to OECD Guideline 301D.
The closed bottle study was started by the direct addition of 5 and 10 mg of test substance to 281 ml capacity test bottles, giving two nominal test concentrations, 17.8 and 35.6 mg/L.
The theoretical oxygen demand (ThOD) of the test substance is 969.7 mg O2/g (assuming no nitrification). At 28 days the oxygen consumptions in the nominal 17.8 and 35.6 mg/L test concentrations were 0.0078 and 0.0080 g O2/g respectively. The percentage degradation was calculated as (BOD/ThOD) x 100.
At day 0 the measured concentrations of F6TF in the nominal 17.8 and 35.6 mg/L test solutions were both 62% of nominal. At day 28 the measured concentrations were 62 and 65% respectively, of nominal.
Samples were incubated in the dark at approximately 20 ºC and analysed at 5 day intervals to determine the biological oxygen demand. Degradation after 28 days was < 11% at the lowest concentration tested (11 mg/L) and < 5.9% at 22 mg/L, indicating that the test substance is not readily biodegradable.
Reference
Measured Dissolved Oxygen (mg O2/L) and Associated Calculated Data
Bottle Contents or Calculated Value |
Day 5 |
Day 10 |
Day 15 |
Day 20 |
Day 28 |
Blank Control, |
9.0 |
9.0 |
7.8 |
7.8 |
7.5 |
9.0 |
8.9 |
8.1 |
7.9 |
7.7 |
|
9.0 |
8.9 |
8.1 |
8.0 |
7.9 |
|
8.9 |
8.9 |
8.1 |
7.9 |
7.3 |
|
9.0 |
8.9 |
7.8 |
7.8 |
7.0 |
|
8.9 |
9.0 |
8.2 |
8.0 |
7.7 |
|
Mean (mg O2/L) |
9.0 |
8.9 |
8.0 |
7.9 |
7.5 |
Calculated Concentration (mg/L) |
18.149 |
18.007 |
18.007 |
18.185 |
18.007 |
5 mg Test Substance per Vessel, |
8.6 |
8.8 |
8.0 |
7.6 |
7.7 |
8.7 |
8.8 |
7.7 |
7.7 |
7.9 |
|
8.7 |
8.8 |
7.9 |
7.7 |
7.8 |
|
Mean (mg O2/L) |
8.7 |
8.8 |
7.9 |
7.7 |
7.8 |
Blank - Mean (mg O2/L) |
<2 |
<2 |
<2 |
<2 |
<2 |
BOD (mg O2/g) |
<110 |
<111 |
<111 |
<110 |
<111 |
% Biodegradation (BOD / ThOD x 100) |
<11% |
<11% |
<11% |
<11% |
<11% |
Calculated Concentration (mg/L) |
36.192 |
35.658 |
35.693 |
35.729 |
35.373 |
10 mg Test Substance per Vessel, |
8.8 |
8.9 |
8.0 |
7.8 |
8.0 |
8.8 |
8.9 |
7.8 |
7.8 |
8.0 |
|
8.8 |
8.9 |
8.0 |
7.8 |
7.9 |
|
Mean (mg O2/L) |
8.8 |
8.9 |
7.9 |
7.8 |
8.0 |
Blank - Mean (mg O2/L) |
<2 |
<2 |
<2 |
<2 |
<2 |
BOD (mg O2/g) |
<55 |
<78 |
<56 |
<56 |
<57 |
% Biodegradation (BOD / ThOD x 100) |
<5.7% |
<8% |
<5.8% |
<5.8% |
<5.9% |
Note A: concentration is calculated from the largest weight used for each set of triplicate bottles divided by the bottle volume, then x 1000
Description of key information
Not readily or inherently biodegradable
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Four biodegradation studies are available and are consistent in quality and results. All studies are K1 and followed OECD guidelines. Zero to 11% of the substance degraded after 28 days, indicating that the test substance is not readily or inherently biodegradable.
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