Registration Dossier
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EC number: 262-797-3 | CAS number: 61444-38-0
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
EC50 (invertebrates)= 6.2 mg/l (95% CL: 5.8-6.4 mg/l) TWA, 48 hour, freshwater, OECD 202, Harlan Laboratories Ltd. 2014
NOEC (invertebrates)= 2.2 mg/l, 48 hour TWA, freshwater, OECD 202, Harlan Laboratories Ltd. 2014
LOEC (invertebrates)= 4 mg/l, 48 hour TWA, freshwater, OECD 202, Harlan Laboratories Ltd. 2014
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 6.2 mg/L
Additional information
The acute toxicity to Daphnia magna was carried out according to OECD 202 Daphnia sp., Acute Immobilisation Test and EU Method C.2 guidelines. Media preparation trials indicated that the use of a saturated solution method of preparation was most appropriate for this test item. Following a preliminary range-finding test using static conditions, a further media preparation trial was completed. Then twenty daphnids (4 replicates of 5 daphnids) were exposed to an aqueous solution of the test item at nominal concentrations of 10, 18, 32, 56 and 100% v/v saturated solution for 48 hours at a temperature of approximately 20 °C under semi-static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 24 and 48 hours. The number of immobilized Daphnia were recorded after 24 and 48 hours. Analysis of the freshly prepared test media for the 32, 56 and 100% v/v saturated solution at 0 and 24 hours showed measured test concentrations to range from 2.7 to 11 mg/L. A decline in measured test concentration was observed in the old or expired test media at 24 and 48 hours to between 1.6 and 7.1 mg/L and hence it was considered appropriate to calculate the results based on the time-weighted mean measured test concentration only in order to give a “worst case” analysis of the data. The study met the acceptability criteria prescribed by the guideline. The 48h-EC50 was 6.2 mg/l (95% CL: 5.8 – 6.4 mg/l) based on time weighted mean measured test concentrations.
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