Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP-Guideline study according to OECD

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report Date:
2005

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Retinol crystalline
- Lot/batch No.: 090-04-06
- Analytical purity: 93.9 %

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
Inoculum: Activated sludge from laboratory wastewater plant treating municipal sewage.
Concentration of activated sludge in the test assay: 30 mg/l (dry substance).
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
24 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
80 - 90
Sampling time:
28 d
Details on results:
Kinetic of test substance (in %):
= 8 after 5 day(s)
= 13 after 7 day(s)
= 52 after 17 day(s)
= 66 after 24 day(s)
= 81 after 28 day(s)


Ten day window failed

BOD5 / COD results

Results with reference substance:
Kinetic of control substance (in %):
= 1 after 3 day(s)
= 70 after 10 day(s)

Applicant's summary and conclusion

Interpretation of results:
other: biodegradable