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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, however only screening test was performed. Non GLP, no chemical analysis and no information given on the purity of the test substance.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Principles of method if other than guideline:
Screening test performed according to the OECD guideline 202
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Retinol crystalline
- Lot/batch No.: CP 090-03-04-57 Fi. 279
No data on test substance purity

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
The highest test concentration, 100 mg test substance/l, was prepared in M4 medium and stirred for approx. 20 hours. Subsequently, the test solution was centrifuged at 17700 G for 20 minutes. Following this, serial dilutions of the aqeous extract (eluate) were prepared in order to obtain the desired test concentrations.

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Nominal and measured concentrations:
Nominal test concentrations (mg/l):
0.01, 0.1, 1, 10, 100, control
Details on test conditions:
TEST SYSTEM
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium according to OECD guideline

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

The number of mobile daphnids after hours of exposure in the different  test concentrations was as following: 

Cumulative immobilization of daphnia after 48 hours [%]:
Nominal test concentration
[mg/l] 48h
0.00 / 0 /
0.01 / 5 /
0.10 / 0 /
1.00 / 10 /
10.00 / 0 /
100.00 / 35 /

Applicant's summary and conclusion