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EC number: 939-595-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- June 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study, according to OECD471.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- octyl phosphonic acid
- IUPAC Name:
- octyl phosphonic acid
- Test material form:
- other: wax
- Details on test material:
- identity of the test material: octyl phosphonic acid
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA 100, TA 1535, TA 1537, TA 1538, TA 98 and E. coli W2uvrA
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Experiment I: 4, 20, 100, 500, 2500 or 10000 µg/plate
Experiment II: 4, 20, 100, 500, 2500 or 5000 µg/plate - Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- benzo(a)pyrene
- other: N-Methyl-N-nitro-N-nitrosoguanidine, 2-Aminoanthracene
- Details on test system and experimental conditions:
- Toxicity experiments and dose range finding:
The first experiment (I) was performed with all tester strains using three plates per dose to get information on mutagenicity and toxicity for calculation of an approriate dose range. A reduced rate of spontaneously occuring colonies as well as visible thinning of the bacterial lawn were used as indicator for toxicity. Thinning of the bacterial lawn was controlled microscopically.
In combination with the second experiment, toxicity testing was performed as follows:
0.1 mL of the different dilutions of the test compound were thoroughly mixed with 0.1 mL of 10exp(-6) dilution of the overnight culture of TA100 and plate with Histidine and Biotin rich top agar (3 plates per dose). The solvent control is compared with the number of colonies per plate in the presence of the tst compound. Results are given as a ration of these values (=surviving fraction).
Mutagenicity test:
Top agar is prepared for the Salmonella strains by mixing 100 mL Agar (0.6 % Agar, 0.5% NaCl) with 10 mL of a 0.5 mL Histidine-biotin solution. Wthi E. coli Histidine is replaced by Tryptophan (2.5 mL, 0.5. mM). The following ingredients are added to 2 mL of molten top agar at 45°C:
0.1 mL of an overnight nutrient broth culture of the bacterial tester strain, 0.1 mL test compound solution, 0.5 mL S9 mix or buffer.
After mixing, the liquid is pooured into a Petridish with minimal Agar (1.2 % Agar, Vogel-Bonner E medium with 2% Glucose). After incubation for approx. 48 hours at 37°C in the dark, colonies are counted. Two independent exeriments were performed.
Positive controls:
Positive control plates were included for each strain. the following substances were used as positive controls:
a) without S9 mix
Sodium azide: TA100, TA 1535
9-Aminocridine: TA1537
2-Nitrofluorene: TA98, TA1538
N-Methyl-N-nitro-N-nitrosoguanidine (MNNG): E. coli WP2uvrA
b) with S9 mix
Benzo[a]pyrene: TA98, TA100, TA1535, TA1537, TA1538, E. coli WP2uvrA
2-Aminoanthracene: TA98, TA100, TA1535, TA1537, TA1538, E. coli WP2uvrA - Evaluation criteria:
- No data
- Statistics:
- not madatory
Results and discussion
Test resultsopen allclose all
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 2500 µg/plate and higher
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium, other: TA 98, TA 100, TA 1535, TA 1537, TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 500 µg/plate and higher
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Controls:
Control plates (background control and positive controls) gave the expected number of colonies.
Toxicity test:
The test compound was tested at doses of 4 to 10000 µg/plate and proved to be toxic to the most of the bacterial strains at doses of 500 or 2500 µg/plate. Thinning of the bacterial lawn and a reduction in the number of colonies have been observed at these doses. Visible precipitation of the test compound on the plates has been observed at 10000 µg/plate.
Mutagencity test:
The test item did not cause a significant increase in the number of revertant colonies with any of the tester strains either in the absence or presence of S9 mix. No dose dependent effect was obtained. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The structural analogue octyl phosphonic acid did not cause any increase in the number of revertant colonies with any of the tester strains in absence or presence of S9 mix. - Executive summary:
The structural analogue octyl phosphonic acid was tested for mutagenicty with the strains TA 100, TA 1535, TA 1537, TA 1538, TA 98 of Salmonella typhimurium and Escherichia coli WP2uvrA (for read across justification please refer to attached document).
The mutagnicity studies were conducted in the absence and in the presence of a metabolising system derived from rat liver homogenate. A dose range of 6 different doses from 4 to 5000 µg/plate was used.
Control plates without mutagen showed that the number of spontaneous revertant colonies was similar to that described in the literature. All positive control comounds gave the expected increase in the number of revertant colonies.
Toxicity: The test item proved to be toxic to the most of the bacterial strains at 500 µg/plate. 5000 µg/plate was chosen as top dose level for the mutagenicity study.
Mutagenicity: In the absence of the metabolic activation system the test compound did not show a dose dependent increase in the number of revertants in any of the bacterial strains. Also in the presence of metabolic activation system, treatment of the cells with
octyl phosphonic acid did not result in relevant increases in the number of revertant colonies.
It can be stated that the structural analogue octyl phosphonic acid is not mutagenic in these bacterial test systems either with or without metabolic activation.
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