Nonamethylenediamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012-10-10 to 2012-10-29

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material:
PHYSICO-CHEMICAL PROPERTIES
- Water solubility: 80 g / 100 g

Vehicle:

yes

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish (Brachgydanio rerio)
- Source: QIAN HU CORPORATION LIMITED, Singapore
- Length at study initiation (length definition, mean, range and SD): Approximate 2.0 ± 1.0 cm
- Feeding during test: no
- Frequency: three times per week until 24 hours before initiation of the exposure. Feed was withdrawn during the exposure period.
- Food type: Super TROPICALBIT

ACCLIMATION
- Acclimation period: 7 days
- Health during acclimation (any mortality observed): No animal was found dead during the acclimatisation period

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

The fish was examined at 3, 6, 24, 48, 72 and 96 hours after dosing. Observation of mortality was conducted at each observation point. In addition to mortalitiy, visible abnormalities of test fish were recorded. Dead fish were moved when observed and mortalities were recorded.

Hardness:

acclimatisation setting-in period: 0 mg/L (GH)
7-day acclimatization period before exposure: 0 mg/L (GH)
during exposure period: 0 - 30 mg/L (GH)

Test temperature:

20.8 - 22.7 °C

pH:

acclimatisation setting-in period: pH 6.50 - 6.57
7-day acclimatization period before exposure: pH 6.85 - 6.96
during exposure period: pH 6.82 - 10.35

Dissolved oxygen:

acclimatisation setting-in period: 94 %
7-day acclimatization period before exposure: 84 - 95 %
during exposure period: 86 - 96 %

Nominal and measured concentrations:

Nominal exposure level of test substance: 90.0; 60.0 ; 40.0 ; 26.7; 17.8; 0 (negative control) mg/L

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: Volume of each test solution 25 L; test item (stock solution): 20 g/L dissolved in distilled water.
- Aeration: was given by air pump
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light / 12 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5
- Range finding study: As stated in the study plan, the concentrations of the test substance for the study were selected based on the results of preliminary range-finding test using zebra fish. In the preliminary range-finding test, four concentrations were used: 100 mg/L (limit concentration), 20 mg/L, 4 mg/L and 0.8 mg/L (geometic interval = 5). In 96-hr exposure period, 100 % fish died at limit concentration 100.0 mg/L; 100 % fish survived at the concentration 20.0 mg/L and below.
- Test concentrations: Based on the results of range-finding test, five concentrations were selected for the study: 90.0 mg/L, 60.0 mg/L, 40.0 mg/L, 26.7 mg/L and 17.8 mg/L.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

40.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Based on the probit analysis of the data, the LC50 value (probit probability = 0.500) with 95 % percent confidence (p = 0.95).

Details on results:

- Behavioural abnormalities: After 3 h with exposure of 40.0 mg/L of the test substance some animals swam slowly; after 3 h with exposure of 60.0 mg/L of the test substance most of animals swam slowly
- Observations on body length and weight: No adverse effect was observed
- Mortality of control: none
- Other adverse effects control: No adverse effect was observed

Reported statistics and error estimates:

The probit analysis was conducted using IBM SPSS Statistics 19.

Sublethal observations / clinical signs:

Probit anlaysis of LC50 value with 95% percent confidence.

The exposure levels were adjusted using the actual concentrations of the test solutions at 96 hr of exposure. The exposure level, total number of animals used in each exposure and cumulative mortality are as below:

Exposure level used for LC50 calculation (mg / L)	Total number of animals used	Cumulative mortality during 96-hr observation	Cumulative mortality (%)
0.0	10	0	0
17.8	10	1	10
26.7	10	0	0
40.0	10	3	30
60.0	10	10	100
90.0	10	10	100

Validity criteria fulfilled:

yes

Conclusions:

Based on the above results, in the acute toxicity study using zebra fish as test system under static conditions, the LC50 of the test item nonamethylenediamine (NMDA) is 40.5 mg/L (probit probability = 0.500) with 95% percent confidence (p = 0.95).

Description of key information

Based on a reliable study according to OECD 203 using zebra fish as test system under static conditions, the LC50 of the test item NMDA is 40.5 mg/L (probit probability = 0.500) with 95% percent confidence (p = 0.95).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

40.5 mg/L