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EC number: 248-502-0 | CAS number: 27503-81-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1971
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1971
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- not specified
- Principles of method if other than guideline:
- study was performed prior to OECD guideline availability but followed in principle the method as described there. The toxicity of the test material after oral administration was studies in Wistar rats. A 16.5% solution of the test item was administered orally (gavage) to 10 animals at a volume of 40 mL/kg body weight (bw) resulting in a dose of 6600 mg/kg. After an observation period of 14 days the animals were killed and investigated for macroscopic signs of pathological changes.
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 2-phenyl-1H-benzimidazole-5-sulphonic acid
- EC Number:
- 248-502-0
- EC Name:
- 2-phenyl-1H-benzimidazole-5-sulphonic acid
- Cas Number:
- 27503-81-7
- Molecular formula:
- C13H10N2O3S
- IUPAC Name:
- 2-phenyl-1H-benzimidazole-5-sulphonic acid
Constituent 1
Test animals
- Species:
- other: mouse and rat
- Strain:
- other: mouse--CF-1; rat--Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Experimental animals and housing conditions
Animals employed in this study were male SPF-bred mice of the strain CF1 with a mean weight of about 20 g and male SPF-bred Wistar rats with a mean weight of 220 g.
The mice had been supplied by Winkelmann - Kirchborchen, the rats by Mus Rattus AG, Brunnthal near Munich. Mice and rats were housed in groups of 5 animals each in Makrolon Type I and Type III cages, respectively, at a room temperature of about 22 °C and natural light.They received pellets of dry feed (supplier, Altromin GmbH, Lage/Lippe) and water ad libitum.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- not specified
- Details on oral exposure:
- Test article-Na solution was administered in its present form as a 16.5% concentration with an application volume of 40 ml/kg body weight, which is, for technical reasons, the maximum volume to be applied. Administration to mice and rats was performed using a gavage. Prior to application the animals were kept non-fasted.
- Doses:
- 6600 mg were administered as a 16,5% Novantisol-Na solution at a volume of 40 ml/kg body weight.
- No. of animals per sex per dose:
- 10 mice; 10 rats
- Control animals:
- not specified
- Details on study design:
- Signs and deaths occurring during a 14-day observation period were recorded in the study records. After the observation period the animals were sacrificed and necropsied.
- Statistics:
- Calculation of the LD50 on the confidence level of p=0.5 is usually performed with the program-controlled probit analysis according to FINK and HUND.
Results and discussion
Effect levels
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 6 600 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 6,600 mg test article-Na was tolerated by the animals investigated without signs.
- Mortality:
- No mortality was observed throughout the observation period (14 days).
- Clinical signs:
- other: Acute toxicity of test article-Na in mice after oral administration: 6,600 mg test article-Na was tolerated by the mice investigated without signs. As the 6,600 mg were administered as a 16.5 % Test article-Na solution at a volume of 40 ml/kg body weight
- Gross pathology:
- Necropsy of the mice sacrificed after a 14-day observation period gave no indication of macroscopically noticeable pathological changes.
Neither did necropsies of rats after 14 days of observation give any indication of pathological organ changes.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- LD50 (oral, rat/mouse): >6600 mg/kg body weight
- Executive summary:
This acute toxicity of test article was performed with mice and rats at a single concentration of 6600 mg/kg body weight by oral gavage. As the 6,600 mg were administered as a 16.5 % Test article-Na solution at a volume of 40 ml/kg body weight and administration of a higher dose was not possible for technical reasons, no lethal dose could be established. Necropsy of the animals sacrificed after a 14-day observation period gave no indication of macroscopically noticeable pathological changes.
In conclusion, LD50 of sodium salt of test article is considered to be greater than 6600 mg/kg body weight.
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