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EC number: 220-482-8 | CAS number: 2781-11-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- (1986)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- (1984)
- Deviations:
- not applicable
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Direct weighings were used to prepare the different test item concentrations.
For the reference compound a stock solution at a concentration of 500 mg/L was prepared by dissolving 500 mg 3.5-Dichlorophenol in 10 mL of 1 M NaOH and diluting to 1 litre with deionised water. The pH was adjusted to pH 7±0.5 with H2SO4 - Test organisms (species):
- activated sludge
- Details on inoculum:
- - mixed population of aquatic microorganisms, origin of a laboratory scale sewage treatment unit (31), treating predominantly industrial sewage.
(Location: Bürrig / Germany))
- pretreatment: aeration of the activated sludge at 20°C ± 2°C, daily fed with synthetic medium
- test concentration of the activated sludge: 6 g/L dry weight
- Preparation of inoculum for exposure:
- first, the sludge was settled and the supernatant was decanted
- after centrifuging (20 min at 4000 rpm and 20°C) the supernatant was decanted
- approx. 1 g of the wet sludge was dried in order to calculate the amount of sludge to achieve a concentration of activated sludge of 6 g/i (dry
weight) suspended solids
- the calculated amount of sludge was first dissolved in synthetic medium and than filled up to a defined end vofume with deionised water
- Before use the pH of the activated sludge was measured and adjusted to pH 6-8. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- no data
- Test temperature:
- 20.4 °C (concerning the highest concentration of 10000 mg/L)
- pH:
- 7.8 (concerning the highest concentration of 10000 mg/L)
- Dissolved oxygen:
- oxygen (mg/L): O2-start 5.3; O2-end 2.9 (concerning the highest concentration of 10000 mg/L)
- Salinity:
- no data
- Nominal and measured concentrations:
- test item: 100, 1000 and 10000 mg/L (nominal)
reference compound: 1 and 20 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL glass Erlenmeyer flasks
- type of application: direct weighing
- temperature: 20 +/- 2 °C
- test concentration: 3 (100, 1000, 10000 mg/L)
- test parameter: respiration rate
- incubationtime: 3 hours with permanent aeration
- Two controls without test item were included in the test design, one at the start and the other at the end of the test series.
- The exposure medium to determine the physico-chemical oxygen consumption was prepared as the medium with the test item, but without activated sludge. - Reference substance (positive control):
- yes
- Remarks:
- 3.5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- See tables in "Any other information on results incl. tables".
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: See table 3 in "Any other information on results incl. tables". 0% and 68.6% inhibition were measured at 1 and 20 mg/L of 3,5-dichlorophenol, respectively. The EC50 of the reference compound is well in the range 5-30 mg/L. - Validity criteria fulfilled:
- yes
- Remarks:
- (respiratory rates of the 2 controls differ less than 15 % from each other)
- Conclusions:
- Diethyl bis(2-hydroxyethyl)-aminomethylphosphonate showed 4% respiration inhibition of activated sludge at the highest test item concentration of 10000 mg/L. The EC5O is > 10000 mg/L.
- Executive summary:
According to ISO 8192 the test substance showed 4% respiration inhibition of activated sludge at the highest test item concentration of 10000 mg/L. The EC5O is > 10000 mg/L. Activated sludge from predominantly domestic sewage plant was exposed to the test solution of three nominal concentrations of the substance (100, 1000, and 10000 mg/L), blank control solution and solution with positive reference substance for a period of 3 hours. Dissolved oxygen was recorded and respiration rates were claculated. No physico-chemical oxygen consumption was observed at the highest test concentration. No respiration inhibition was observed at 100 and 1000 mg/L; and only 4 % inhibition at 10000 mg/L. This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to microoragnisms.
Reference
Table 1: Oxygen content, temperature and pH-values during exposure phase
| Test item concentration (mg/L) | O2 start (mg O2/L) | O2 end (mg O2/L) | Time (start-end) (min.) | Temperature (°C) | pH |
Test item | 100 | 5.20 | 2.40 | 5.0 | 20.4 | 8.0 |
1000 | 5.90 | 2.70 | 6.0 | 20.4 | 8.0 | |
10000 | 5.30 | 2.90 | 5.0 | 20.4 | 7.8 | |
Control 1 Control 2 |
| 5.00 5.80 | 2.50 4.80 | 5.0 2.0 | 20.0 20.4 | 7.9 8.0 |
Physico-chemical oxygen consumption control | 10000 | 9.00 | 9.00 | 10.0 | 20.4 | 8.0 |
3,5-dichlorophenol | 1 | 4.10 | 2.00 | 4.0 | 20.0 | 7.9 |
20 | 7.60 | 6.50 | 7.0 | 20.2 | 7.9 |
Table 2: Results of test item
Nominal test item concentration (mg/L) | Respiratory rate (mg/L.h) | % inhibition |
Control mean | 30.0 | -- |
100 | 33.6 | 0 |
1000 | 32.0 | 0 |
10000* | 28.0 | 4 |
Comments: Test concentrations are given as nominal concentrations and were not confirmed by analytical methods.
* The physico-chemical oxygen consumption has been determined at 10000 mg/L test item concentration. As no physico-chemical oxygen consumption was observed at that test item concentration this observation also holds true for the lower test item concentrations.
Table 3: Results of the reference compound 3,5-dichlorophenol
Nominal reference compound concentration (mg/L) | Respiratory rate (mg/L.h) | % inhibition |
Control mean | 30.0 | -- |
1 | 31.5 | 0 |
20 | 9.4 | 68.6 |
Description of key information
Diethyl bis(2-hydroxyethyl)-aminomethylphosphonate showed 4% respiration inhibition of activated sludge at the highest test item concentration of 10000 mg/L (Müller, 1990). The EC5O was > 10000 mg/L.
Key value for chemical safety assessment
Additional information
A reliable, guideline, non GLP study is available, and therefore considered as key.
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