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EC number: 218-254-8 | CAS number: 2094-98-6
One valid experiment was performed.
The test item1,1'-Azobis(cyclohexane-1-carbonitrile)was applied to a three-dimensional human cornea tissue model in duplicate for an exposure time of 6 hours.
The solid test item was applied to each tissue.
After treatment, the respective substance was rinsed from the tissue; then, cell viability of the tissues was evaluated by addition of MTT, which can be reduced to formazan. The formazan production was evaluated by measuring the optical density (OD) of the resulting solution.
Demineralised water was used as negative control and methyl acetate was used as positive control.
The controls showed the following results: After treatment with the negative control, the absorbance values were within the required acceptability criterion of mean OD>0.8 and < 2.5, OD was 1.5. The positive control showed clear eye irritating effects, mean value of the relative tissue viability was 32.4 % (< 50 %).
The variation within tissue replicates was acceptable (< 20 %).
After treatment with the test item, the mean value of relative tissue viability was 115.5 %.
This value is well above the threshold for eye irritation potential (≤ 60 %).
Under the conditions of the test,1,1'-Azobis(cyclohexane-1-carbonitrile)is considered non- eye irritant in the EpiOcularTMEye Irritation Test.
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