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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
other: Literature
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Ethylisothiocyanate - MUTAGENICITY OF ISOTHIOCYANATES, ISOCYANATES AND THIOUREAS ON SALMONELLA TYPHIMURIUM
Author:
YAMAGUCHI T
Year:
1980
Bibliographic source:
AGRIC BIOL CHEM 44:3017-3018,1980
Reference Type:
publication
Title:
MUTAGENICITY TEST OF FOOD ADDITIVES WITH SALMONELLA TYPHIMURIUM TA97 AND TA102
Author:
FUJITA,H, AOKI,N AND SASAKI,M
Year:
1994
Bibliographic source:
TOKYO-TORITSU EISEI KENKYUSHO KENKYU NENPO 45:191-199

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethyl isothiocyanate
EC Number:
208-831-2
EC Name:
Ethyl isothiocyanate
Cas Number:
542-85-8
Molecular formula:
C3H5NS
IUPAC Name:
ethyl isothiocyanate
Test material form:
liquid

Method

Species / strainopen allclose all
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Species / strain / cell type:
S. typhimurium TA 1538
Species / strain / cell type:
S. typhimurium, other: TA 1536
Metabolic activation:
with and without
Metabolic activation system:
S9 mix
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified

Results and discussion

Test resultsopen allclose all
Key result
Species / strain:
S. typhimurium TA 1535
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid
Key result
Species / strain:
S. typhimurium TA 1537
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid
Key result
Species / strain:
S. typhimurium TA 1538
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid
Key result
Species / strain:
S. typhimurium TA 98
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid
Key result
Species / strain:
S. typhimurium TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid
Key result
Species / strain:
S. typhimurium, other: TA 1536
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid

Applicant's summary and conclusion

Conclusions:
The test substance (ethylisothiocyanate) does not induce mutations in bateria with or without metabolic activation.
Executive summary:

The test substance (ethylisothiocyanate) does not induce mutations in bateria with or without metabolic activation.