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EC number: 202-908-4 | CAS number: 101-02-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- endocrine system modulation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 28.11.2012 - 05.12.2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: recent study conducted according to reliable in-house protocol
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Method performed according to P. Sohoni and J.P. Sumpter (1998)
The aim of the study was to determine the androgenic and antiandrogenic potential of the test item. The yeast cells used in the yeast androgen screening (YAS) assay have been stably transformed with genes for the human androgen receptor and the ß-galactosidase reporter gene. Androgenic as well as antiandrogenic potentials of the test substances mediated by receptor binding or blocking can be determined using this assay, respectively. - GLP compliance:
- no
- Type of method:
- in vitro
Test material
- Reference substance name:
- Triphenyl phosphite
- EC Number:
- 202-908-4
- EC Name:
- Triphenyl phosphite
- Cas Number:
- 101-02-0
- Molecular formula:
- C18H15O3P
- IUPAC Name:
- triphenyl phosphite
- Details on test material:
- - Name of test material: Triphenyl phosphite
- Physical state: Liquid, yellowish
- Storage condition of test material: Room temperature
Constituent 1
Test animals
- Species:
- other: Yeast
- Details on test animals or test system and environmental conditions:
- The yeast strain was cultivated by orbital shaking (85 rpm) at approximately 32°C for 24 - 72 h. For this 100 ml culture medium was inoculated with a thawed aliquot of yeast stock culture. Optical density (OD) was measured at 690 nm and the test culture prepared. As a rule, 0.5 ml of the preculture with an OD of 1.0 was transferred into 50 ml fresh growth medium including 0.5 ml chromogenic substrate CPRG (chlorophenolred-ß-D-galactopyranoside).
Administration / exposure
- Route of administration:
- other: in vitro application
- Vehicle:
- DMSO
- Duration of treatment / exposure:
- 48h
- Frequency of treatment:
- Once
Doses / concentrations
- Remarks:
- 10E-10, 10E-9, 10E-8, 10E-7, 10E-6, 10E-5, 10E-4 mol/L
- Control animals:
- yes
- Details on study design:
- The study was carried out in 96-well microtiter plates in which 2 µL of different test substance concentrations were pipetted. Afterwards 200 µL of the test culture containing the chromogenic substrate CPRG were added to each well, the plates were sealed with adhesive tape and incubated on an orbital shaker at about 32°C until measurement of the OD. After 48 h (+/- 4 h) incubation, absorbance of the plates was measured at 570 nm (color development, androgen receptor dependent enzyme expression) and 690 nm (turbidity due to growth of the yeast).
Examinations
- Examinations:
- Evaluation was performed by calculating the difference of the measured ODs at the two wavelengths (absorption at 570 nm - absorption at 690 nm).
- Positive control:
- Yes
- Androgenic positive control: Dihydrotestosterone (10E -11, 10E-10, 10E-9, 5*10E-9, 10E-8, 10E-7, 10E-6 mol/L)
- Antiandrogenic positive control: Dihydrotestosterone (5*10E-9 mol/L combined with hydroxyflutamide 10E-5 mol/L)
Results and discussion
- Details on results:
- ln this study, the test substance did not show androgenic activity in comparison to dihydrotestosterone. ln this study, the test substance showed clear antiandrogenic activity at 10E-05 mol/L in comparison to hydroxyflutamide. No cytotoxicity was observed.
Applicant's summary and conclusion
- Conclusions:
- Based on the results of this study, the test substance showed no androgenic activity in comparison to dihydrotestosterone. ln this study, the test substance showed clear antiandrogenic activity at 10E-05 mol/L in comparison to hydroxyflutamide. No cytotoxicity was observed.
- Executive summary:
The yeast strain was cultivated by orbital shaking (85 rpm) at approximately 32°C for 24 - 72 h. For this 100 ml culture medium was inoculated with a thawed aliquot of yeast stock culture. Optical density (OD) was measured at 690 nm and the test culture prepared. As a rule, 0.5 ml of the preculture with an OD of 1.0 was transferred into 50 ml fresh growth medium including 0.5 ml chromogenic substrate CPRG (chlorophenolred-ß-D-galactopyranoside). The study was carried out in 96-well microtiter plates in which 2 µL of different test substance concentrations were pipetted. Afterwards 200 µL of the test culture containing the chromogenic substrate CPRG were added to each well, the plates were sealed with adhesive tape and incubated on an orbital shaker at about 32°C until measurement of the OD. After 48 h (+/- 4 h) incubation, absorbance of the plates was measured at 570 nm (color development, androgen receptor dependent enzyme expression) and 690 nm (turbidity due to growth of the yeast). Evaluation was performed by calculating the difference of the measured ODs at the two wavelengths (absorption at 570 nm - absorption at 690 nm). Each experiment includes negative controls (vehicle controls) and positive controls for the verification of the androgenic (dihydrotestosterone 10 -11, 10-10, 10-9, 5*10-9, 10-8, 10-7, 10-6 mol/L) and antiandrogenic (dihydrotestosterone 5*10-9 mol/L combined with hydroxyflutamide 10-5 mol/L) activity of the yeast cells.
Final concentration of test material [mol/L]: 10-10, 10-9, 10-8, 10-7, 10-6, 10-5, 10-4
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