Triphenyl phosphite

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Triphenyl phosphite

EC Number:

202-908-4

EC Name:

Triphenyl phosphite

Cas Number:

101-02-0

Molecular formula:

C18H15O3P

IUPAC Name:

triphenyl phosphite

Details on test material:

- Name of test material (as cited in study report): Triphenyl Phosphit
- Physical state: clear colorless liquid

Method

Species / strain

Species / strain / cell type:

other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98, TA 100

Metabolic activation:

with and without

Metabolic activation system:

Aroclor 1254 induced S-9 fraction from rat liver

Test concentrations with justification for top dose:

5, 10, 50, 100 and 500 µg/plate

Vehicle / solvent:

ethanol

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: see below

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation)

DURATION
- Exposure duration: 48h at 37°C

NUMBER OF REPLICATIONS:
Samples are run in duplicate

POSITIVE CONTROLS
- with metabolic activation:
TA-1535, cyclophosphamide (200 µg/plate); TA-1537, TA-1538, TA-98 and TA-100, benzofalpyrene 5 µg/plate

- without metabolic activation:
TA-1535 and TA-100, sodium azide (1 µg/plate); TA-1537, aaminoacridine (100 µg/plate); TA-1538 and TA-98, 2-nitrofuorene (50 µg/plate)

Evaluation criteria:

The assay is scored as the ratio of the number of macroscopic colonies on the test plate over the number of macroscopic colonies on the control plate. This is taken as the mutagenic index. A compound is considered to have a positive response if the mutagenic index is 2.0 and the apparent mutagenicity exhibits a dose response relationship.

Results and discussion

Test results

Species / strain:

other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98, TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Additional information on results:

- All strains/cell types tested

TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: A white precipitate formed in the aqueous agar solution at 50, 100 and 500 µg/plate

Applicant's summary and conclusion

Conclusions:

The test article was not mutagenic under the conditions of this study described.