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EC number: 500-740-9 | CAS number: 162492-07-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From Septeber 03, 2017 to Septeber 08, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- see 'Principles of method if other than guideline'
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- yes
- Remarks:
- see 'Principles of method if other than guideline'
- Principles of method if other than guideline:
- The following deviation from the study plan was documented:
- Temperature during husbandry was higher than stated in the guideline. As no mortality occurred during husbandry at least 12 days before the start of the test and as no mortality was observed in the control during the test this was stated as uncritical. The deviation was and assessed signed by the study director on 12. Oct. 2017. - GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- CAS No.: 162492-07-1; EINECS-No.: 500-740-9; Batch No.: 1591ZG-101; Purity: >98 %; Appearance: homogeneous, white powder
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Specification: Species Danio rerio HAMILTON-BUCHANAN, Age: sexually immature young fish, length: 2 +/- 1 cm, Origin: The animals were obtained from a commercial supplier. Before being used for the test, the fish were kept for 12 days under test conditions. During this period, mortality did not surpass 5 %. Supplier: Dehner Garten Center, 67433 Neustadt.
- Test type:
- semi-static
- Water media type:
- other: Good quality drinking water is used.
- Remarks:
- The composition is analysed yearly by the supplier.
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 0.83 mmol/L
- Test temperature:
- 22.9 – 23.3 °C
- pH:
- 7.9 - 8.2
- Dissolved oxygen:
- 7.7 - 9.0 mg/L
- Conductivity:
- 228 μS/cm at 25 °C
- Nominal and measured concentrations:
- Treatment: 100 mg/L nominal concentration.
The measured concentration in the new solutions was 123.36 μg/L and 137.27 μg/L and in the old solutions 62.58 μg/L and 50.73 μg/L. Therefore, the determination of the results was based on the geometric mean of the measured concentrations. - Details on test conditions:
- Medium renewal: after 48 hours
Duration: 96 hours
Loading: 1 fish/L
Vessels: glass aquaria, volume 10 L
Aeration: accomplished with glass tubes
Feeding: none
Photo period: 12/12 hours using neon tubes
Replicate (Treatments): 1 vessel, each containing 7 L test solution and 7 fish
Replicate (Blank Control): 1 vessel, containing 7 L tap water and 7 fish
Observations were made every 24 hours, meaning measuring pH and O2-concentration of the old and new test solution in each vessel and documenting mortalities or abnormal behaviour. The content of the test substance in the test vessels was measured at the beginning and at the end of the test and at medium renewal after 48 hours in the old and the new solutions. In the control the content of the test substance was measured at the beginning and at the end of the test. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: corresponding to >85.63 µg/L mean measured
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: corresponding to 85.63 µg/L mean measured
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 96 h LC50 and NOEC were determined to be > 100 and 100 mg/L, corresponding to >85.63 and 85.63 µg/L, respectively.
- Executive summary:
A study was conducted to determine the toxicity of the test substance to Zebra fish (Danio rerio) according to OECD Guideline 203 and EU Method C.1, in compliance with GLP. The study was performed using one concentration of the test substance (100 mg/L, nominal) in a semi-static design. One vessel was used for the treatment containing 7 L of test solution and 7 fish. Similarly, one vessel was used for the blank control containing 7 L of tap water and 7 fish. Observations were made every 24 h. The concentration of the substance in the vessels was measured at the beginning and at the end of the test and at medium renewal after 48 h in the old and the new solutions using HPLC. At the tested concentration, no mortality was observed. Due to poor solubility, the measured concentrations were in the low range: 123.36 and 137.27 μg/L in the new solutions, and 62.58 and 50.73 μg/L in the old solutions. Under the study conditions, the 96 h LC50 and NOEC were determined to be > 100 and 100 mg/L, corresponding to >85.63 and 85.63 µg/L, respectively (Muckle, 2017).
Reference
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 85.63 µg/L
Additional information
A study was conducted to determine the toxicity of the test substance to Zebra fish (Danio rerio) according to OECD Guideline 203 and EU Method C.1, in compliance with GLP. The study was performed using one concentration of the test substance (100 mg/L, nominal) in a semi-static design. One vessel was used for the treatment containing 7 L of test solution and 7 fish. Similarly, one vessel was used for the blank control containing 7 L of tap water and 7 fish. Observations were made every 24 h. The concentration of the substance in the vessels was measured at the beginning and at the end of the test and at medium renewal after 48 h in the old and the new solutions using HPLC. At the tested concentration, no mortality was observed. Due to poor solubility, the measured concentrations were in the low range: 123.36 and 137.27 μg/L in the new solutions, and 62.58 and 50.73 μg/L in the old solutions. Under the study conditions, the 96 h LC50 and NOEC were determined to be > 100 and 100 mg/L, corresponding to >85.63 and 85.63 µg/L, respectively (Muckle, 2017).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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