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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: read across from similar substance
Adequacy of study:
key study
Study period:
1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Sufficiently well documented study according to national guideline.
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
- Preparation of a stock solution of 120 mg/L in water
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM- Common name: Daphnia magna Strauss- Strain: not available- Source: Bayer AG Leverkusen- Age at study initiation (mean and range, SD): 6-24 hrs- Food type: Scenedesmus Subspicatus (ad libitum)- Frequency: Daily- Health during acclimation (any mortality observed): none- Cultivation: daily feeding with Scenedesmus Subspicatus (ad libitum) (SOP 4003)- Water : "aufbereitetes Leverkusener Trinkwasser" of drinking quality.- Medical pre-treatment: none
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Nominal and measured concentrations:
Nominal test concentrations: 0.32, 1.0, 3.2, 10, 31.6 and 100 mg/L
Details on test conditions:
- Test vessels (cylindrical) : 4 cm diameter and 7 cm high- Volume of the test medium: 20 mL- Ventilation: none- Reference substance: Potassium dichromate- Measurement and numerical values during the test: Oxygen, pH, Temperature of water, Number of immobile Daphnia
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
0.32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
0.57 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Results with reference substance (positive control):
- Results with reference substance valid? yes
Validity criteria fulfilled:
yes
Conclusions:
The EC50 (24 h) of the substance to Daphnia magna was determined to be 0.57 mg/L.
Executive summary:

The 24–hr-acute toxicity of the test item to Daphnia magna was studied at nominal concentrations of 0.32, 1.0, 3.2, 10, 31.6 and 100 mg/L. Test conditions were similar to the standard test guideline EU Method C.2 (Acute toxicity for Daphnia).

The 24– hour EC50was determined to be 0.57 mg/L. 

A classification for aquatic toxicity based on the results of this study is not possible as the test duration is too short (24hr instead of 48hr).

Description of key information

EC50 = 0.57 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.57 mg/L

Additional information

The short term toxicity to aquatic invertebrates was determined using the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test).
Based on the results, the classification in Aquatic Acute and Chronic 1 is required by CLP Regulation.