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Ecotoxicological information

Short-term toxicity to fish

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Link to relevant study record(s)

Description of key information

Determination of the acute fish toxicity on Rainbow trout with FAT 40863/A TE.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

This study was performed to assess the acute toxicity of the test item to rainbow trout (Oncorhynchus mykiss). The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No 203, "Fish, Acute Toxicity Test" referenced as Method C.1 of Commission Regulation (EC) No. 440/2008.



Following a preliminary range-finding test, fish were exposed, in groups of seven, to an aqueous solution of the test item, at a single concentration of 100 mg/L for a period of 96 hours at a temperature of approximately 14 ºC under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 1, 3 and
6 hours after the start of exposure and then daily throughout the test until termination after
96 hours.



Analysis of the test preparations at 0, 24, 72 and 96 hours showed measured test concentrations to be near nominal and so it was considered justifiable to estimate the LC50values in terms of the nominal test concentrations.

Exposure of rainbow trout (Oncorhynchus mykiss)to the test item gave LC50values based on the nominal test concentrations of greater than 100 mg/L. The No Observed Effect Concentration was 100 mg/L.

It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/L.