Undec-10-enal

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

This study was designed to assess the toxic effects of the test compound on the Zebra fish (Danio rerio). Bowl aquaria containing 10 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance was insoluble in water while soluble in organic solvent but after adding water in the organic solvent the substance reappear. Therefore, the test substance was taken as it is & solution was prepared by dissolving 8 µl, 17.6 µl, 38.7 µl, 85.1 µl & 187.2 µl of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 0.8 mg/L, 1.76 mg/L, 3.87 mg/L, 8.51 mg/L & 18.72 mg/L, respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra Fish
- Source:Orange City Aquarium, Sitabuldi, Nagpur, MS (India)
- Age at study initiation (mean and range, SD):3-4 months
- Length at study initiation (length definition, mean, range and SD):average 2.42 cm
- Weight at study initiation (mean and range, SD):0.117 g (average)
- Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
- Feeding during test
- Food type:Standard brand feed
- Frequency:once daily

ACCLIMATION
- Acclimation period:15 days
- Acclimation conditions (same as test or not):Fishes, to be used in the study, are kept in the test water for seven days immediately before testing
- Type and amount of food:Standard brand feed
- Feeding frequency:Once in a day up to sixth day of acclimatization
- Health during acclimation (any mortality observed):

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

82.5 mg of CaCO3

Test temperature:

24-28°C

pH:

6.62

Dissolved oxygen:

no data available

Salinity:

no data available

Nominal and measured concentrations:

0.8 mg/L, 1.76 mg/L, 3.87 mg/L, 8.51 mg/L & 18.72 mg/L, respectively.

Details on test conditions:

TEST SYSTEM
- Test vessel: The test vessels used were all glass bowl aquaria of 12 inch each.
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness

- Range finding study:
Available data from previously conducted studies by different scientists (Ref.1) and predictions of LC50 values from computational programs (Ref.2) suggested the adverse effects of the test substance at lower concentrations. Hence, a range finding test was conducted with the several test concentrations. The test concentrations selected for the study were 0.8 mg/L, 1.76 mg/L, 3.87 mg/L, 8.51 mg/L & 18.72 mg/L. Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment.

- Test concentrations: 0.8 mg/L, 1.76 mg/L, 3.87 mg/L, 8.51 mg/L & 18.72 mg/L.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

8.51 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No abnormal effects on swimming and no mortality were found

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 18.72 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Other details not known

Details on results:

After 72 hrs of exposure period mortality in 3 fishes were observed.

LC100 (96 hours) (lowest concentration at which 100% mortality was observed) = 100% mortality was not observed in any of the 5 concentrations.
Determination of mortality and symptoms: Determination or calculation of the median lethal concentration (LC 50) and the symptoms of the nominal concentrations were done after 24, 48, 72 and 96 hours.

Sublethal observations / clinical signs:

OBSERVATIONS:

 

Table 1. Mortality and symptoms

Nominal conc. (mg/l)	Number of fish	Mortality (cumulated) after
		24h	48h	72h	96h
0 (control)	8	-	-	-	-
0.8	8	-	-	-	-
1.76	8	-	-	-	-
3.87	8	-	-	-	-
8.51	8	-	-	-	-
18.72	8	-	-	3	3

 

Table 2: Visible Symptoms: No abnormal symptoms observed

 

Nominal conc. (mg/l)	Number of fish	Symptoms after
		24h	48h	72h	96h
0 (control)	8	NS8	NS8	NS8	NS8
0.8	8	NS8	NS8	NS8	NS8
1.76	8	NS8	NS8	NS8	NS8
3.87	8	NS8	NS8	NS8	NS8
8.51	8	NS8	NS8	NS8	NS8
18.72	8	NS8	NS8	NS5	NS5

 

 

 

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

 

 

 

 Table 3. pH and oxygen content:

Nominal conc. (mg/l)	pH readings after
	24h	48h	72h	96h
0 (control)	7.6	7.6	7.7	7.8
0.8	7.7	7.6	7.8	7.9
1.76	7.6	7.6	7.7	8.0
3.87	7.7	7.7	7.6	8.2
8.51	7.7	7.7	7.4	8.0
18.72	7.8	7.7	7.5	7.4

 

 

Nominal conc. (mg/l)	Oxygen content (mg/l) after
	24h	48h	72h	96h
0 (control)	6.60	6.67	6.50	6.83
0.8	6.41	6.3	6.10	6.66
1.76	6.45	5.99	5.80	6.45
3.87	6.38	5.9	5.7	6.34
8.51	6.65	5.70	5.65	5.92
18.72	6.62	6.19	5.99	5.87

 

 

Table 4: Test temperature

Nominal conc. (mg/l)	Temperature (°C) after
	24h	48h	72h	96h
0 (control)	25.0	26.2	26.1	22.7
0.8	25.1	27.2	27.1	23.0
1.76	25.1	27.7	27.5	23.3
3.87	25.0	27.8	27.6	23.8
8.51	24.9	28.0	27.9	24.0
18.72	24.8	28.4	27.0	26.0

 

 

Validity criteria fulfilled:

not specified

Conclusions:

Based on nominal concentrations, experimental no lethal concentration [LC-0 (96 h)] for the test chemical on Danio rerio was observed to be at 8.51 mg/L. Mortality were observed at the concentration > 18.72 mg/l.

Executive summary:

Short term aquatic toxicity study (96 hrs) was conducted to assess the toxic effects of the test compound. The study was conducted according to the OECD guideline 203 on Zebra fish (Danio rerio) in a fresh water static system at a nominal concentration level of 0.8 mg/L, 1.76 mg/L, 3.87 mg/L, 8.51 mg/L for 96 hrs. Observations for mortality, visible symptoms, pH, Temperature, dissolved oxygen content were recorded after 24 hours, 48 hours, 72 hours and 96 hours after the start of the experiment. No mortality was recorded in any of the concentration upto 8.51 mg/l and the fishes were freely swimming without showing any abnormal symptoms. No mortalities were found in the control aquaria.  Based on nominal concentrations, experimental no lethal concentration [LC-0 (96 h)] for the test chemical on Danio rerio was observed to be at 8.51 mg/L. Mortality were observed at the concentration > 18.72 mg/l. Thus based on the LC50 value, chemical consider to be toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

.

Description of key information

Short term aquatic toxicity study (96 hrs) was conducted to assess the toxic effects of the test compound. The study was conducted according to the OECD guideline 203 on Zebra fish (Danio rerio) in a fresh water static system at a nominal concentration level of 0.8 mg/L, 1.76 mg/L, 3.87 mg/L, 8.51 mg/L for 96 hrs. Observations for mortality, visible symptoms, pH, Temperature, dissolved oxygen content were recorded after 24 hours, 48 hours, 72 hours and 96 hours after the start of the experiment. No mortality was recorded in any of the concentration upto 8.51 mg/l and the fishes were freely swimming without showing any abnormal symptoms. No mortalities were found in the control aquaria.  Based on nominal concentrations, experimental no lethal concentration [LC-0 (96 h)] for the test chemical on Danio rerio was observed to be at 8.51 mg/L. Mortality were observed at the concentration > 18.72 mg/l. Thus based on the LC50 value, chemical consider to be toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

18.72 mg/L

Additional information

Summarized result for the toxicity of test chemical and structurally and functionally similar read across chemicals on the mortality of fishes were reviewed and mention as below:

 

Short term aquatic toxicity study (96 hrs) was conducted to assess the toxic effects of the test compound. The study was conducted according to the OECD guideline 203 on Zebra fish (Danio rerio) in a fresh water static system at a nominal concentration level of 0.8 mg/L, 1.76 mg/L, 3.87 mg/L, 8.51 mg/L for 96 hrs. Observations for mortality, visible symptoms, pH, Temperature, dissolved oxygen content were recorded after 24 hours, 48 hours, 72 hours and 96 hours after the start of the experiment. No mortality was recorded in any of the concentration upto 8.51 mg/l and the fishes were freely swimming without showing any abnormal symptoms. No mortalities were found in the control aquaria.  Based on nominal concentrations, experimental no lethal concentration [LC-0 (96 h)] for the test chemical on Danio rerio was observed to be at 8.51 mg/L. Mortality were observed at the concentration > 18.72 mg/l. Thus based on the LC50 value, chemical consider to be toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

 

Similarly in the second data for read across chemical the acute toxicity of industrial organic chemicals including test chemical to the fathead minnow was determined for 96 hrs. Concentrations were determined by analytical methods utilizing direct aqueous injection and solvent extraction gas chromatographic (GC) techniques, and high pressure liquid chromatographic (HPLC) analyses. GC analyses were performed on a Hewlett-Packard model 5730A gas chromatograph equipped with flame ionization and electron capture detectors. Tests were conducted in diluters with continuous-flow water delivery and toxicant introduction systems. Each of two diluters for binary tests contained a control, and four treatment levels in duplicate at each of seven mixture ratios were distributed between the diluters. This gave a total of 28 treatments in duplicate plus two controls. The seven ratios used to define the binary isobole diagrams were 5:0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:5. The diluter for equitoxic multiple chemical tests had one control and five single treatment levels. The toxicant concentrations followed a geometric series (0.8 dilution factor) for all tests. The dilution water was fed from a main head tank to a similar tank over each diluter. The water in the head tanks was vigorously aerated to remove excess dissolved gases. The toxicant solutions for binary tests were delivered by FMI metering pumps from separate stock bottles or chemical 'saturators' into a chamber which was designed to dilute each stock solution independently before combining them in mixtures. The toxicant concentrations in each test treatment were thus controlled separately. Test chambers were illuminated with wide spectrum fluorescent bulbs for 16 h daily. This included a 30-rain gradual brightening and dimming period with incandescent light to simulate dawn and dusk. The light intensity at the test water surface ranged from 22 to 38 lumens/ sq ft. 95% confidence intervals were computed by the Trimmed Spearman-Karber Method or a log-probit method. From experimental result the the lethal concentration in 96 hrs exposure period was observed to be 45.9 mg/l with 95% confidence limit (43.5-48.4 mg/l).Thus based on the result it is concluded that the test substance is toxic to fish and thus classified in aquatic chronic 3 as per the CLP criteria.

Thus based on the above values, chemical consider to be toxic and classified as aquatic chronic 3 as per the CLP classification criteria.