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EC number: 297-701-9 | CAS number: 93686-48-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: ready biodegradability test OECD 301B : CO2 Evolution (Modified Sturm Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- The medium was prepared from the stock solutions. The stock solution of the positive control was prepared and its TOC was measured. The inoculum was taken from its source, washed, aerated and the dry matter was determined.
The test vessels were filled with medium and inoculum. Then all flasks were aerated for 72 hours with purified, CO2-free, moistened air to purge the system of CO2.
Experimental Parameters
Flask volume 1500 mL
Positive control flasks: 2, containing positive control, mineral medium and inoculum
Toxicity control: 1, containing test item, positive control, mineral medium and inoculum
Inoculum concentration: 24.9 mg/L
Temperature 20.0 – 21.6 °C
Duration 28 days
The test was performed with a nominal start concentration of 20 mg organic carbon/L.
In the positive control flasks, 25.9 mg/L of Aniline were added.
In the toxicity control flask, 25.9 mg/L of Aniline and 27.3 mg/L of phosphorous acid, tri-C12-C14-alkyl esters were added - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge from a biologic sewage treatment plant was used. The chosen plant is treating mostly domestic sewage.
Source: The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf.
Date of collection: 30. Mar 2012, batch no: 20120330.
Pre-Treatment: The sludge was filtrated, washed with tap water twice, then washed with and re-suspended in test medium. It was then aerated for 12 hours. The dry matter was determined with 3740 mg suspended solids/litre - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 28 d
- Test temperature:
- 20.0 – 21.6 °C
- pH:
- 7.41
- Nominal and measured concentrations:
- Nominal concentration: 27.3 mg/L
- Details on test conditions:
- The test vessels were aerated with purified (by activated charcoal), CO2-scrubbed, moistened air. The scrubbing of carbon dioxide was achieved by bubbling the purified air through a flask containing 1.5 m-NaOH. To control the absence of CO2, the air was then led through a flask containing a solution of Ba(OH)2 before reaching the test vessels.
Magnetic stirrers were used to prevent deposition of inoculum.
The emitted CO2 was trapped in 0.25-m-NaOH. Two scrubbers containing 100 mL each were connected in series to the test vessels. The initial IC value of the 0.25 m-NaOH was separately determined in each flask.
From each front scrubber flask, ten samples were taken in order to determine the emitted CO2. (on days 0, 2, 4, 7, 9, 11, 14, 18, 23 and 29). The sample volume was 1 mL. The resulting change in the volume of the front flask was considered in the calculation of emitted CO2.
On day 28, 5 mL HCl 2-m. were added to each test flask in order to drive off dissolved CO2. On day 29, samples from both scrubber flasks were taken.
Analyses of the emitted CO2 were made by IC measurement using the carbon analyser TOC multi N/C 2100S, Analytik Jena. - Reference substance (positive control):
- yes
- Remarks:
- Aniline
- Duration:
- 29 d
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 27.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The degradation observed in the toxicity flask was 66.4% at the end of the test (29 days). This degradation is very closed to the degradation percentage observed in the positive controls (68.7% at day 29) containing Aniline alone, without test substance. Therefore, it can be stated that the test item is not toxic towards the inoculum in a concentration of 27.3 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- NOEC (29 d) = 27.3 mg/L (highest and only dose tested)
- Executive summary:
The toxicity toward the microorganisms of the phosphorous acid, triC12-C14 alkylesters was investigated in a GLP-compliant ready biodegradability screening study performed in accordance with OECD Guideline No. 301B via the toxicity control flask. The phosphorous acid, triC12-C14 alkylesters was not found to be toxic to microorganisms under the test conditions of the readily biodegradation test.
The NOEC (29 d) was 27.3 mg/L corresponding to the highest and only dose tested.
Reference
In the following table, the calculated emitted carbon (in mg/L) is presented:
Day |
Control 1 |
Control 2 |
Toxicity Control |
2 |
0.42 |
0.57 |
0.86 |
4 |
0.98 |
1.28 |
8.74 |
7 |
1.50 |
1.80 |
12.54 |
9 |
1.98 |
2.05 |
21.10 |
11 |
2.34 |
2.66 |
25.42 |
14 |
2.56 |
3.01 |
30.16 |
18 |
2.63 |
3.11 |
28.41 |
23 |
3.04 |
3.65 |
32.50 |
29 |
3.14 |
3.95 |
30.33 |
In the following table, the percentage biodegradation is presented:
Day |
Positive Control 1 |
Positive Control 2 |
Positive Control Mean |
Toxicity Control |
0 |
0.0 |
0.0 |
0.0 |
0.0 |
2 |
-0.2 |
1.1 |
0.5 |
0.9 |
4 |
16.6 |
10.0 |
13.3 |
18.9 |
7 |
49.8 |
50.5 |
50.1 |
27.0 |
9 |
60.8 |
61.8 |
61.3 |
47.3 |
11 |
68.5 |
68.5 |
68.5 |
56.8 |
14 |
77.3 |
74.7 |
76.0 |
67.9 |
18 |
75.9 |
73.4 |
74.7 |
63.4 |
23 |
79.3 |
81.3 |
80.3 |
72.3 |
29 |
68.7 |
68.3 |
68.5 |
66.4 |
Because of a poor calibration, the calculated degradation of the positive control and in the toxicity flask on day 23 were slightly higher than on day 29. This can be stated as uncritical for the outcome of the study.
No observations were made which might cause doubts concerning the validity of the study outcome.
The result of the test can be considered valid.
Description of key information
The toxicity toward the microorganisms of the phosphorous acid, triC12-C14 alkylesters was investigated in a GLP-compliant ready biodegradability screening study performed in accordance with OECD Guideline No. 301B via the toxicity control flask. The phosphorous acid, triC12-C14 alkylesters was not found to be toxic to microorganisms under the test conditions of the readily biodegradation test.
The NOEC (29 d) was 27.3 mg/L corresponding to the highest and only dose tested.
Key value for chemical safety assessment
Additional information
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