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EC number: 235-469-2
CAS number: 12237-63-7
This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 45160:2.
Short term toxicity of test material
was evaluated on aquatic invertebrate Daphnia magna for 24 hr according
to EEC-Guideline L251 .Cultures of daphnia were maintained in glass
vessels containing approx. 2.5 1 of reconstituted waterat 20 ± 1"C
(water is renewed partially thrice weekly). At each renewal the daphnia
are fed a suspension of green algae (Scenedesmus subspicatus)
supplemented by a suspension of TETRAMIN-extract in such quantities that
the food is consumed after 24h. In the pretest 24h before the start of
the test reproductive daphnia were separated from the young by sieving
all individuals through a 800 µm seive. This operation is repeated 6h
before the start of the test and the young (6-24h of age) are retained
for the test. Test was performed in beaker covered with watch glass
having fill volume of 100 ml. The nominal concentrations at which test
was performed were 0.0010, 0.0018, 0.0032, 0.0058, 0.010 0.018, 0.032,
0.058, 0.10, 0.18, 0.32 0.58, 1.0 mg/l. The EC50-values were calculated
according to SPEARMAN-KAERBER in: D.J. Finney, 524-530, London (1964).
EC-values were graphically determined on gausso-logarithmic probability
paper. The effective concentration (EC50) value of test material in
aquatic invertebrate (Daphnia Magna) in a 24 hr study on the basis of
mobility was estimated to be 0.023 mg/l. Based on the above effect
concentration it can be considered that the test material as toxic to
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