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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
December 1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to guideline
Guideline:
other: EEC-Guideline L251 Vol. 27, 84/449/EEC: C.
Deviations:
yes
Remarks:
No stock solution was prepared; Fish size: 20-26 mm (average: 23 mm).
Principles of method if other than guideline:
The LC-50 values were calculated according to the maximum likelihood method, probit model (Mc Cullagh, P., Nelder, J.A., 1983: Generalized linear models, Chapinan & Hall, London)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
No stock solution was prepared. The test substance was mixed directly in the water of the tanks
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Number of fishes : 10 fishes per concentration and control 10 fishes per aquarium
Length . 23 mm (20-26 mm)
Weight : 0.15 g (0.08-0.19 g)
Loading : 0.10 g/l
Feeding : None
Adaptation : 24 hours/no food 24 hours prior to exposure
Acclimatisation : 26 days
Treatment : None
Supplier: West Aquarium 1 D-3422 Bad Lauterberg
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
160 ing CaCO3/L
Test temperature:
22 ± 10°C
Nominal and measured concentrations:
Test concentrations (mg/l) : 1.0, 1.8, 3.2, 5.8, 10
Remarks (nominal) : Calculated amounts of test substance to produce the desired test concentrations were given into the water and were homogeneously distributed. The fish were then transfered into the aquarla.
Controls: Blank: water
Details on test conditions:
Water : Dechlorinated tap water (carbon filter)
Aeration : none
Light : Fluorescent ijyht, 16 hours daily
Measurements : oxygen, pH, temperature daily
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
6.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 4.6-9.4 mg/l
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
52 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
51 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
> 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
LC 50 (96h) graphically determined 5.6 mg/L
LC 0 (96h) in test 1.8 mg/L
LC 100 (96h) in test : >10 mg/L

Controls : Mortalities in blank : 0 %
Sublethal observations / clinical signs:

MEASUREMENTS / OBSERVATIONS

Table 1: Mortalities

Concentration

nominal (mg/L)

Mortalities

Number of dead fish

24 h                         48 h                        72 h                96 h

Blank

0

0

0

0

1.0

0

0

0

0

2.0

0

0

0

0

3.0

0

0

0

1

4.0

0

2

4

6

5.0

0

1

1

7

 

Conclusions:
The lethal concentration (LC50) value of test material in zebra fish in a 96 hr study was observed to be 6.3 mg/l.
Executive summary:

Short term toxicity to fish for 96 h was evaluated for the test material . The test was performed as per EEC-Guideline L251.No stock solution was prepared.The test substance was mixed directly in the water of the tanks .10 fishes per concentration and control were used .Glass aquaria of 20 liters filled with 15 liters Dechlorinated tap water (carbon filter) was used as medium . The nominal test concentration used were 1.0, 1.8, 3.2, 5.8, 10 mg/l . The LC-50 values were calculated according to the maximum likelihood method, probit model. The lethal concentration (LC50) value of test material in zebra fish in a 96 hr study was observed to be 6.3 mg/l. Based on the above effect concentration it can be concluded that test substance was toxic for fish and hence, considered to be classified in aquatic chronic category 2 as per CLP criteria.

Description of key information

Short term toxicity to fish:

Short term toxicity to fish for 96 h was evaluated for the test material . The test was performed as per EEC-Guideline L251.No stock solution was prepared.The test substance was mixed directly in the water of the tanks .10 fishes per concentration and control were used .Glass aquaria of 20 liters filled with 15 liters Dechlorinated tap water (carbon filter) was used as medium . The nominal test concentration used were 1.0, 1.8, 3.2, 5.8, 10 mg/l . The LC-50 values were calculated according to the maximum likelihood method, probit model. The lethal concentration (LC50) value of test material in zebra fish in a 96 hr study was observed to be 6.3 mg/l. Based on the above effect concentration it can be concluded that test substance was toxic for fish and hence, considered to be classified in aquatic chronic category 2 as per CLP criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
6.3 mg/L

Additional information

Short term toxicity to fish:

Short term toxicity to fish for 96 h was evaluated for the test material . The test was performed as per EEC-Guideline L251.No stock solution was prepared.The test substance was mixed directly in the water of the tanks .10 fishes per concentration and control were used .Glass aquaria of 20 liters filled with 15 liters Dechlorinated tap water (carbon filter) was used as medium . The nominal test concentration used were 1.0, 1.8, 3.2, 5.8, 10 mg/l . The LC-50 values were calculated according to the maximum likelihood method, probit model. The lethal concentration (LC50) value of test material in zebra fish in a 96 hr study was observed to be 6.3 mg/l. Based on the above effect concentration it can be concluded that test substance was toxic for fish and hence, considered to be classified in aquatic chronic category 2 as per CLP criteria.